Transcript: Human XM_017003752.1

PREDICTED: Homo sapiens cilia and flagella associated protein 65 (CFAP65), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CFAP65 (255101)
Length:
6154
CDS:
241..6063

Additional Resources:

NCBI RefSeq record:
XM_017003752.1
NBCI Gene record:
CFAP65 (255101)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003752.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000154042 CGGCAGATTTATGAGCTGTAT pLKO.1 4192 CDS 100% 4.950 6.930 N CFAP65 n/a
2 TRCN0000427079 TGGAATGAGCAGTCAACTAAG pLKO_005 5416 CDS 100% 10.800 8.640 N CFAP65 n/a
3 TRCN0000153970 CGACTACTTTCTGGCTAACTT pLKO.1 5109 CDS 100% 5.625 4.500 N CFAP65 n/a
4 TRCN0000250335 CGGCAACTGCACCCTCTATTA pLKO_005 3297 CDS 100% 13.200 9.240 N Cfap65 n/a
5 TRCN0000424790 GAGAATCCATCAGTCTCATTT pLKO_005 289 CDS 100% 13.200 9.240 N CFAP65 n/a
6 TRCN0000154072 CCTCAACAACATCTCCAAGAA pLKO.1 4623 CDS 100% 4.950 3.465 N CFAP65 n/a
7 TRCN0000158264 CCTGCTGGAAGACAAGAACTT pLKO.1 5340 CDS 100% 4.950 3.465 N CFAP65 n/a
8 TRCN0000152736 CAGCCTGTACTTAATGCCAAT pLKO.1 5454 CDS 100% 4.050 2.835 N CFAP65 n/a
9 TRCN0000158071 CTTTGGGAATGTGCTGGTGAA pLKO.1 3240 CDS 100% 4.050 2.835 N CFAP65 n/a
10 TRCN0000153853 CCTGTACTTAATGCCAATCCT pLKO.1 5457 CDS 100% 3.000 2.100 N CFAP65 n/a
11 TRCN0000157716 CCTGGTCAACAAAGACTGCAA pLKO.1 2637 CDS 100% 2.640 1.848 N CFAP65 n/a
12 TRCN0000419125 GGCAACTGCACCCTCTATTAC pLKO_005 3298 CDS 100% 13.200 7.920 N CFAP65 n/a
13 TRCN0000165520 GAGGAGGAAGAAGAGGAGAAA pLKO.1 5620 CDS 100% 4.950 2.475 Y AP5B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003752.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14451 pDONR223 100% 6.9% 6.1% None (many diffs) n/a
2 ccsbBroad304_14451 pLX_304 0% 6.9% 6.1% V5 (many diffs) n/a
3 TRCN0000472983 CTCAAAAAATGCACATTCAGGTCC pLX_317 79.1% 6.9% 6.1% V5 (many diffs) n/a
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