Construct: ORF TRCN0000472983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017905.1_s317c1
- Derived from:
- ccsbBroadEn_14451
- DNA Barcode:
- CTCAAAAAATGCACATTCAGGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP65 (255101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255101 | CFAP65 | cilia and flagella associat... | NM_152389.4 | 98.7% | 97.5% | (many diffs) |
| 2 | human | 255101 | CFAP65 | cilia and flagella associat... | NM_001278296.2 | 18% | 16.2% | (many diffs) |
| 3 | human | 255101 | CFAP65 | cilia and flagella associat... | NM_001278295.1 | 16.8% | 15.1% | (many diffs) |
| 4 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510919.1 | 7.9% | 7.1% | (many diffs) |
| 5 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510916.1 | 7.5% | 6.5% | (many diffs) |
| 6 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510911.1 | 7.1% | 6.4% | (many diffs) |
| 7 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510912.1 | 7.1% | 6.4% | (many diffs) |
| 8 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510914.1 | 7.1% | 6.4% | (many diffs) |
| 9 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510915.1 | 7.1% | 6.4% | (many diffs) |
| 10 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_017003754.1 | 7.1% | 6.4% | (many diffs) |
| 11 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_017003755.1 | 7.1% | 6.4% | (many diffs) |
| 12 | human | 255101 | CFAP65 | cilia and flagella associat... | NM_194302.4 | 6.9% | 6.2% | (many diffs) |
| 13 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510910.1 | 6.9% | 6.2% | (many diffs) |
| 14 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_017003753.1 | 6.9% | 6.2% | (many diffs) |
| 15 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510908.1 | 6.9% | 6.1% | (many diffs) |
| 16 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510909.1 | 6.9% | 6.1% | (many diffs) |
| 17 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_017003752.1 | 6.9% | 6.1% | (many diffs) |
| 18 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510903.1 | 6.9% | 6.1% | (many diffs) |
| 19 | human | 255101 | CFAP65 | cilia and flagella associat... | XM_011510904.2 | 6.9% | 6.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cacccaggct tcaagctccg tcgtgaggtc caggaacagc aggaaccaca 121 ccgtgaactc tggtggatcc tgcctgagtg ccagcacagt ggCCATCCCT GCCATCAACG 181 ACAGCAGTGC AGCCATGAGT GCCTGCAGCA CCATCAGCGC CCAGCCCGCA AGCTCCATGG 241 ACACTCAGAT GCACTCCCCA AAGAAGCAGG AGAGAGTGAA CAAGAGGGTC ATCTGGGGCA 301 TTGAGGTGGC TGAGGAGCTG CATTGGAAAG GCTGGGAGCT AGGAAAGGAG ACCACAAGGA 361 ATCTGGTTCT GAAAAATCGA TCCTTGAAAC TCCAGAAGAT GAAGCACAGG TACCAGTATA 421 AGGGATCAAG GACCCAGTGC CACAGCCTTG AGCCCCGAAA GCAGGCTCTC TTCAAAACAA 481 AACAAAACAA ACAAAAAAAA CCTTTAACAT GCCATATAAA AGCCAGTGAG TGTTTAAAAT 541 ACATGCAGTA TGAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTCAAAAAAT GCACATTCAG 721 GTCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt