Transcript: Human XM_017005643.2

PREDICTED: Homo sapiens nischarin (NISCH), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NISCH (11188)
Length:
4075
CDS:
148..3489

Additional Resources:

NCBI RefSeq record:
XM_017005643.2
NBCI Gene record:
NISCH (11188)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017005643.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256839 ACTCTAGTCGCGTCAAGTTTA pLKO_005 2906 CDS 100% 13.200 18.480 N NISCH n/a
2 TRCN0000583848 CCTCTCTAACCAAGGAATCAT pLKO_005 489 CDS 100% 5.625 7.875 N NISCH n/a
3 TRCN0000162814 CGACTACAACAACAGCCCTTT pLKO.1 2610 CDS 100% 4.050 5.670 N NISCH n/a
4 TRCN0000256840 ACAGGCAGCTTCCGATGATTT pLKO_005 624 CDS 100% 13.200 10.560 N NISCH n/a
5 TRCN0000256843 CTGTTGTGGGACCGTTGTTAA pLKO_005 3746 3UTR 100% 13.200 9.240 N NISCH n/a
6 TRCN0000163146 GCTGAGAACCGCTACTTTGAA pLKO.1 922 CDS 100% 5.625 3.938 N NISCH n/a
7 TRCN0000162969 GCCTGTTGACAAGGACTTCTA pLKO.1 2832 CDS 100% 4.950 3.465 N NISCH n/a
8 TRCN0000163059 CCAGGGATGTTCTGATTCCTT pLKO.1 585 CDS 100% 3.000 2.100 N NISCH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017005643.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07768 pDONR223 100% 73.9% 73.8% None 0_1ins1173;1608G>C;1994C>T n/a
2 ccsbBroad304_07768 pLX_304 0% 73.9% 73.8% V5 0_1ins1173;1608G>C;1994C>T n/a
3 TRCN0000476629 CCAAAACAGGGGTCACAGGAATCA pLX_317 8.3% 73.9% 73.8% V5 0_1ins1173;1608G>C;1994C>T n/a
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