Transcript: Human XM_017006390.1

PREDICTED: Homo sapiens TAFA chemokine like family member 1 (TAFA1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TAFA1 (407738)
Length:
3085
CDS:
1134..1532

Additional Resources:

NCBI RefSeq record:
XM_017006390.1
NBCI Gene record:
TAFA1 (407738)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017006390.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168845 CCAGATAATCACAGTGCGTTT pLKO.1 1651 3UTR 100% 4.050 5.670 N TAFA1 n/a
2 TRCN0000166897 CCTACATGAATAAGTCCTGTA pLKO.1 2362 3UTR 100% 4.050 3.240 N TAFA1 n/a
3 TRCN0000172421 CACTCCCTGACAATTCTGGAT pLKO.1 1456 CDS 100% 2.640 1.848 N TAFA1 n/a
4 TRCN0000172965 GAACAACAAGAAACCGGCCTT pLKO.1 1357 CDS 100% 2.160 1.512 N TAFA1 n/a
5 TRCN0000168747 GAAGAATGTAAGACACTCCCT pLKO.1 1443 CDS 100% 0.660 0.462 N TAFA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017006390.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05659 pDONR223 100% 99.2% 99.2% None 116_117insAGA n/a
2 ccsbBroad304_05659 pLX_304 0% 99.2% 99.2% V5 116_117insAGA n/a
3 TRCN0000477047 TTTTCGCTTCACATCCCCTAGCAG pLX_317 91.1% 99.2% 99.2% V5 116_117insAGA n/a
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