Construct: ORF TRCN0000477047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014357.1_s317c1
- Derived from:
- ccsbBroadEn_05659
- DNA Barcode:
- TTTTCGCTTCACATCCCCTAGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA1 (407738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 407738 | TAFA1 | TAFA chemokine like family ... | NM_001252216.1 | 100% | 100% | |
| 2 | human | 407738 | TAFA1 | TAFA chemokine like family ... | NM_213609.4 | 100% | 100% | |
| 3 | human | 407738 | TAFA1 | TAFA chemokine like family ... | XM_017006386.1 | 100% | 100% | |
| 4 | human | 407738 | TAFA1 | TAFA chemokine like family ... | XM_017006387.2 | 100% | 100% | |
| 5 | human | 407738 | TAFA1 | TAFA chemokine like family ... | XM_017006388.1 | 99.2% | 99.2% | 116_117insAGA |
| 6 | human | 407738 | TAFA1 | TAFA chemokine like family ... | XM_017006389.1 | 99.2% | 99.2% | 116_117insAGA |
| 7 | human | 407738 | TAFA1 | TAFA chemokine like family ... | XM_017006390.1 | 99.2% | 99.2% | 116_117insAGA |
| 8 | mouse | 320265 | Fam19a1 | family with sequence simila... | NM_182808.3 | 90.9% | 99.2% | (many diffs) |
| 9 | mouse | 320265 | Fam19a1 | family with sequence simila... | XM_017321638.1 | 77% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatggtctct gcgatgtcct gggTCCTGTA TTTGTGGATA AGTGCTTGTG 121 CAATGCTACT CTGCCATGGA TCCCTTCAGC ACACTTTCCA GCAGCATCAC CTGCACAGAC 181 CAGAAGGAGG GACGTGTGAA GTGATAGCAG CACACCGATG TTGTAACAAG AATCGCATTG 241 AGGAGCGGTC ACAAACAGTA AAGTGTTCCT GTCTACCTGG AAAAGTGGCT GGAACAACAA 301 GAAACCGGCC TTCTTGCGTC GATGCCTCCA TAGTGATTGG GAAATGGTGG TGTGAGATGG 361 AGCCTTGCCT AGAAGGAGAA GAATGTAAGA CACTCCCTGA CAATTCTGGA TGGATGTGCG 421 CAACAGGCAA CAAAATTAAG ACCACGAGAA TTCACCCAAG AACCTACCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA TTTTCGCTTC ACATCCCCTA GCAGACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt