Transcript: Human XM_017009435.2

PREDICTED: Homo sapiens chondroitin sulfate synthase 3 (CHSY3), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CHSY3 (337876)
Length:
2208
CDS:
358..1707

Additional Resources:

NCBI RefSeq record:
XM_017009435.2
NBCI Gene record:
CHSY3 (337876)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017009435.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000158945 GAAGAGTTTCTTAGATCGCTA pLKO.1 1171 CDS 100% 2.640 3.696 N CHSY3 n/a
2 TRCN0000161827 CCACATATTGGTGAATGCCTT pLKO.1 1336 CDS 100% 2.640 2.112 N CHSY3 n/a
3 TRCN0000094036 CGACGACGATGTCTACATCAA pLKO.1 1137 CDS 100% 4.950 3.465 N Chsy3 n/a
4 TRCN0000163437 GCCACATATTGGTGAATGCCT pLKO.1 1335 CDS 100% 0.750 0.525 N CHSY3 n/a
5 TRCN0000094038 GTCCTTCATGATGATCAAGTA pLKO.1 1071 CDS 100% 4.950 2.970 N Chsy3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017009435.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05426 pDONR223 100% 47.8% 42% None (many diffs) n/a
2 ccsbBroad304_05426 pLX_304 0% 47.8% 42% V5 (many diffs) n/a
3 TRCN0000475886 CTGCCCGACCATCTTTTATCCTTC pLX_317 14.1% 47.8% 42% V5 (many diffs) n/a
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