Construct: ORF TRCN0000475886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001198.1_s317c1
- Derived from:
- ccsbBroadEn_05426
- DNA Barcode:
- CTGCCCGACCATCTTTTATCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHSY3 (337876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | NM_175856.5 | 100% | 100% | |
| 2 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_011543363.3 | 64.9% | 64.9% | 0_1ins927 |
| 3 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_011543364.2 | 58.9% | 58.9% | 0_1ins1086 |
| 4 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_017009434.1 | 58.9% | 58.9% | 0_1ins1086 |
| 5 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_017009435.2 | 47.8% | 42% | (many diffs) |
| 6 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_011543365.3 | 44.3% | 41.8% | (many diffs) |
| 7 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_005271982.4 | 42.2% | 41.2% | (many diffs) |
| 8 | human | 337876 | CHSY3 | chondroitin sulfate synthase 3 | XM_005271983.4 | 41.1% | 41% | 1087_1088insTGCAACAACT;1089_1090ins1547 |
| 9 | mouse | 78923 | Chsy3 | chondroitin sulfate synthase 3 | NM_001081328.1 | 87% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2715
- ORF length:
- 2646
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtgcgc tctcgccgcc cgtggatgag cgtggcatta gggctggtgc 121 tgggcttcac cgccgcgtcc tggctcatcg cccccagggt ggcggagctg agcgagagga 181 agagacgtgg ctccagcctc tgctcctact acggtcgctc tgctgctggc ccccgcgccg 241 gcgctcagca gccgctcccc cagccccagt cccgaccacg gcaggagcag tcgccgcccc 301 ccgcgcgcca ggatctccag gggccaccgc tgcccgaggc agcacccggg atcaccagtt 361 ttcgaagcag cccctggcag cagccacctc cgctgcagca gcggcggcga ggacgcgagc 421 ctgagggcgc gacggggctt cccggtgctc cagcggccga gggggagccc gaggaggagg 481 acgggggcgc ggctgggcag cggagagacg gccggccggg gagtagccac aacggcagcg 541 gggacggggg cgctgccgcc ccgagcgccc gaccccggga cttcctgtac gtgggggtga 601 tgaccgcgca gaagtacctg ggcagccgcg cgctggccgc gcagcggacc tgggcgcgtt 661 tcatcccggg ccgcgtggag ttcttttcca gccagcagcc ccccaacgcc ggccagcccc 721 cgccacccct gcctgtcatc gcgctaccgg gtgtggacga ctcctatcct ccccagaaaa 781 agtccttcat gatgatcaag tacatgcacg accactacct ggacaagtat gagtggttca 841 tgcgcgccga cgacgatgtc tacatcaaag gtgataaatt agaagagttt cttagatcgc 901 taaacagcag taagcctctc tacctgggac agactggcct ggggaatatt gaagagcttg 961 gaaagctggg actggagcct ggggaaaact tctgtatggg aggacctggc atgatcttta 1021 gccgagaagt tctcaggagg atggtgccac atattggtga atgccttaga gaaatgtaca 1081 cgactcatga ggatgtggaa gtaggaagat gcgttcgccg ttttggtggg actcagtgtg 1141 tctggtctta cgagatgcaa caactgttcc atgaaaatta tgaacacaat cggaagggtt 1201 acatccaaga ccttcacaat agcaaaatcc atgcagccat aacacttcat cccaacaaaa 1261 ggcctgcata ccaatacagg ctgcataatt acatgctcag ccgcaaaatt tctgaacttc 1321 gctaccgcac catccagctc cacagggaaa gtgccctgat gagcaagctc agtaacacag 1381 aagtgagcaa agaggaccag cagctgggag tgataccttc tttcaaccac ttccagcctc 1441 gggagagaaa tgaagtgata gaatgggagt tcctgacagg gaagcttcta tactcagcag 1501 ctgagaacca gccccctcga cagagcctca gtagcatttt aagaacagca ctggatgata 1561 ccgtcctaca ggtgatggag atgatcaatg agaatgccaa gagcagagga cggctcattg 1621 acttcaagga aattcagtat ggctaccgca gagttaaccc catgcacggg gtggagtaca 1681 ttttggattt actcctttta tacaaaagac acaagggaag gaaactgact gtgccagtga 1741 gacgtcatgc ctatcttcag cagttgttca gcaagccttt cttcagagag accgaagagc 1801 tagatgtcaa cagtcttgtg gagagtatta acagtgaaac tcagtcattc tcctttatat 1861 ctaattcttt aaagatatta tcttcttttc aaggtgccaa agaaatggga gggcacaatg 1921 aaaagaaagt acacattctc gttcctctca tcggaaggta tgacattttc ttgagattca 1981 tggagaactt tgaaaacatg tgtcttatcc caaagcagaa tgtaaagttg gtcattatcc 2041 ttttcagtag ggattctggc caagactcca gcaagcatat tgagctgata aaagggtacc 2101 agaacaagta ccccaaagca gaaatgaccc tgatcccaat gaagggagag ttttccagag 2161 gtcttggtct tgaaatggct tctgcccagt ttgacaatga cactttgctg ctattttgtg 2221 atgttgactt gatcttcaga gaagattttc tccaacgatg tagagacaat acaattcagg 2281 gacaacaggt gtactatccc atcatcttta gccagtatga cccaaaggta acaaacgggg 2341 gaaatccTCC CACTGATGAT TACTTCATAT TCTCAAAAAA GACTGGATTT TGGAGAGACT 2401 ATGGATATGG CATCACCTGT ATTTACAAAA GTGATCTTCT AGGTGCAGGT GGATTTGATA 2461 CCTCAATACA AGGCTGGGGA CTAGAAGATG TAGATCTCTA CAATAAAGTC ATTCTATCTG 2521 GCTTAAGGCC ATTCAGAAGC CAAGAAGTAG GAGTGGTGCA TATTTTCCAT CCAGTTCATT 2581 GTGATCCTAA CTTGGACCCT AAGCAGTATA AGATGTGCTT AGGATCCAAG GCAAGTACTT 2641 TCGCCTCAAC CATGCAACTG GCTGAACTCT GGCTTGAAAA ACATTTAGGT GTCAGGTACA 2701 ATCGAACTCT CTCCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2761 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2821 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGCCCGACC ATCTTTTATC 2881 CTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt