Transcript: Human XM_017009491.1

PREDICTED: Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NDUFS4 (4724)
Length:
488
CDS:
54..410

Additional Resources:

NCBI RefSeq record:
XM_017009491.1
NBCI Gene record:
NDUFS4 (4724)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017009491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000344753 TCAAGACACACAACTCATAAC pLKO_005 194 CDS 100% 10.800 8.640 N NDUFS4 n/a
2 TRCN0000036633 CAACTCATAACAGTTGATGAA pLKO.1 204 CDS 100% 0.495 0.347 N NDUFS4 n/a
3 TRCN0000036632 GAGGACTTCCACATGGAGATT pLKO.1 158 CDS 100% 4.950 2.970 N NDUFS4 n/a
4 TRCN0000333324 GAGGACTTCCACATGGAGATT pLKO_005 158 CDS 100% 4.950 2.970 N NDUFS4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017009491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06625 pDONR223 100% 57.4% 30.9% None (many diffs) n/a
2 ccsbBroad304_06625 pLX_304 0% 57.4% 30.9% V5 (many diffs) n/a
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