Transcript: Human XM_017012102.2

PREDICTED: Homo sapiens acyloxyacyl hydrolase (AOAH), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AOAH (313)
Length:
5938
CDS:
347..1999

Additional Resources:

NCBI RefSeq record:
XM_017012102.2
NBCI Gene record:
AOAH (313)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012102.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050061 CCATCAGGATTCAAACTGTAA pLKO.1 1018 CDS 100% 4.950 6.930 N AOAH n/a
2 TRCN0000050058 CGGCCAGCTAAATAAAGACAT pLKO.1 1576 CDS 100% 4.950 3.960 N AOAH n/a
3 TRCN0000371260 AGACTCTGAAGCATCTAAATT pLKO_005 1464 CDS 100% 15.000 10.500 N AOAH n/a
4 TRCN0000050060 CCAACCATTGTGTCATCTCTA pLKO.1 703 CDS 100% 4.950 3.465 N AOAH n/a
5 TRCN0000050059 GCAGATGTCTTTGAACTCTTT pLKO.1 1180 CDS 100% 4.950 3.465 N AOAH n/a
6 TRCN0000050062 GCAGTTGTACTCCTTCCTGAA pLKO.1 1606 CDS 100% 4.050 2.835 N AOAH n/a
7 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 4058 3UTR 100% 4.950 2.475 Y ORAI2 n/a
8 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 4055 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012102.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05828 pDONR223 100% 95.5% 95.4% None 66G>A;1057_1058ins75 n/a
2 ccsbBroad304_05828 pLX_304 0% 95.5% 95.4% V5 66G>A;1057_1058ins75 n/a
3 TRCN0000473373 GAATTAGGATAACCACCATAATCT pLX_317 30.9% 95.5% 95.4% V5 66G>A;1057_1058ins75 n/a
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