Construct: ORF TRCN0000473373

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009113.1_s317c1
Derived from:
ccsbBroadEn_05828
DNA Barcode:
GAATTAGGATAACCACCATAATCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AOAH (313)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473373
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 313 AOAH acyloxyacyl hydrolase NM_001637.4 99.9% 100% 66G>A
2 human 313 AOAH acyloxyacyl hydrolase XM_017012102.2 95.5% 95.4% 66G>A;1057_1058ins75
3 human 313 AOAH acyloxyacyl hydrolase XM_011515336.2 95% 93.4% (many diffs)
4 human 313 AOAH acyloxyacyl hydrolase NM_001177507.1 94.3% 94.4% 66G>A;126_127ins96
5 human 313 AOAH acyloxyacyl hydrolase XM_011515338.2 92.7% 92.6% (many diffs)
6 human 313 AOAH acyloxyacyl hydrolase XM_017012104.1 88.4% 88.1% (many diffs)
7 human 313 AOAH acyloxyacyl hydrolase XM_011515339.2 88.4% 88.8% (many diffs)
8 human 313 AOAH acyloxyacyl hydrolase XM_011515340.2 88.2% 88.1% 66G>A;1522_1523insTCATAC;1524_1525ins195
9 human 313 AOAH acyloxyacyl hydrolase NM_001177506.1 83.5% 77% 66G>A;1600_1718del;1845_2064del
10 human 313 AOAH acyloxyacyl hydrolase XM_011515333.2 79.8% 73.3% (many diffs)
11 human 313 AOAH acyloxyacyl hydrolase XM_011515334.2 78.8% 72.5% (many diffs)
12 human 313 AOAH acyloxyacyl hydrolase XM_011515335.2 77.5% 70.9% (many diffs)
13 human 313 AOAH acyloxyacyl hydrolase XM_017012106.1 76.2% 75.8% (many diffs)
14 human 313 AOAH acyloxyacyl hydrolase XM_011515341.2 75.9% 75.6% (many diffs)
15 human 313 AOAH acyloxyacyl hydrolase XM_017012105.1 47.5% 38.7% (many diffs)
16 human 313 AOAH acyloxyacyl hydrolase XM_011515342.2 42.9% 37% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1791
ORF length:
1725
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gtccccctgg aaaatcctta cggtggcgcc tctattcttg ctcctgtctc
121ttcagtcctc agcctctcca gccaacgatg accagtccag gcccagcctc tcgaatgggc
181acacctgtgt agggtgtgtg ctggtggtgt ctgtaataga acagcttgct caagttcaca
241actcgacggt ccaggcctcg atggagagac tgtgcagcta cctgcctgaa aaactgttct
301tgaaaaccac ctgctattta gtcattgaca agtttggatc agacatcata aaactgctta
361gcgcagatat gaatgctgat gtggtatgtc acactctgga gttttgtaaa cagaacactg
421gccaaccatt gtgtcatctc taccctcttc ccaaggagac atggaaattt acactacaga
481aggcaagaca aattgtcaag aagtccccga ttctgaaata ttctagaagt ggttctgaca
541tttgttcact cccggttttg gccaagatct gccagaaaat taaattagct atggaacagt
601ctgtgccatt caaagatgtg gattcagaca aatacagcgt tttcccaaca ctgcggggct
661atcactggcg ggggagagac tgtaatgaca gcgacgagtc agtgtaccca ggtagaaggc
721cgaacaactg ggatgtccat caggattcaa actgtaatgg catttggggt gtcgatccaa
781aagatggagt tccatatgag aagaaattct gtgaaggttc acagcccagg ggaatcattt
841tgctgggaga ctcagctggg gctcattttc acatctctcc tgaatggatc acagcgtcgc
901agatgtcttt gaactctttc atcaatctac caacagccct taccaacgag cttgactggc
961cccaactctc tggtgctaca ggatttctgg actccactgt tggaattaaa gaaaaatcta
1021tttaccttcg cttatggaaa agaaaccact gtaatcacag ggactaccag aatatttcaa
1081gaaatggtgc atcttcccga aacctgaaga aatttataga aagcttgtct agaaacaagg
1141tgttggacta tcccgccatc gttatatatg ccatgattgg aaatgatgtc tgcagtggga
1201agagtgaccc agtcccagcc atgaccactc ctgagaaact ctactccaac gtcatgcaga
1261cTCTGAAGCA TCTAAATTCC CACCTGCCCA ATGGCAGCCA TGTTATTTTG TATGGCTTAC
1321CAGATGGAAC CTTTCTCTGG GATAATTTGC ACAACAGATA TCATCCTCTC GGCCAGCTAA
1381ATAAAGACAT GACCTATGCG CAGTTGTACT CCTTCCTGAA CTGCCTCCAG GTCAGCCCCT
1441GCCACGGCTG GATGTCTTCC AACAAGACGT TGCGGACTCT CACTTCAGAG AGAGCAGAGC
1501AACTCTCCAA CACACTGAAA AAAATTGCAG CCAGTGAGAA ATTTACAAAC TTCAATCTTT
1561TCTACATGGA TTTTGCCTTC CATGAAATCA TACAGGAGTG GCAGAAGAGA GGCGGACAGC
1621CCTGGCAGCT CATCGAGCCC GTGGATGGAT TCCACCCCAA CGAGGTGGCT TTGCTGTTGT
1681TGGCGGATCA TTTCTGGAAA AAGGTGCAGC TCCAGTGGCC CCAAATCCTG GGAAAGGAGA
1741ATCCGTTCAA CCCCCAGATT AAACAGGTGT TTGGAGACCA AGGCGGGCAC TGCCCAACTT
1801TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1861CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1921CTTGTGGAAA GGACGAGAAT TAGGATAACC ACCATAATCT ACGCGTTAAG TCgacaatca
1981acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)