Transcript: Human XM_017012303.1

PREDICTED: Homo sapiens ankyrin repeat and SOCS box containing 4 (ASB4), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ASB4 (51666)
Length:
2152
CDS:
534..1640

Additional Resources:

NCBI RefSeq record:
XM_017012303.1
NBCI Gene record:
ASB4 (51666)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012303.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000118389 CCCGAGATGACGACTTTAAAT pLKO.1 1273 CDS 100% 15.000 21.000 N ASB4 n/a
2 TRCN0000118387 GCCAAGTTAGTTAAGAGAAAT pLKO.1 573 CDS 100% 13.200 9.240 N ASB4 n/a
3 TRCN0000118388 GCCTGCCATTCTTGTCCTAAA pLKO.1 1521 CDS 100% 10.800 7.560 N ASB4 n/a
4 TRCN0000118391 CCTCCATCTCTCTGTCTTGTT pLKO.1 767 CDS 100% 4.950 3.465 N ASB4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012303.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03361 pDONR223 100% 91.8% 89.6% None (many diffs) n/a
2 ccsbBroad304_03361 pLX_304 0% 91.8% 89.6% V5 (many diffs) n/a
3 TRCN0000468196 TGGTTGACACCCCCACGCTGCTTC pLX_317 22% 91.8% 89.6% V5 (many diffs) n/a
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