Transcript: Human XM_017013450.2

PREDICTED: Homo sapiens methionine sulfoxide reductase A (MSRA), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MSRA (4482)
Length:
1277
CDS:
193..702

Additional Resources:

NCBI RefSeq record:
XM_017013450.2
NBCI Gene record:
MSRA (4482)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017013450.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000046455 GCAACAGAACAGTCGAACCTT pLKO.1 155 5UTR 100% 3.000 4.200 N MSRA n/a
2 TRCN0000286336 GCAACAGAACAGTCGAACCTT pLKO_005 155 5UTR 100% 3.000 4.200 N MSRA n/a
3 TRCN0000293702 TCGCTGAAATCCTTCGCTTTA pLKO_005 1109 3UTR 100% 10.800 8.640 N MSRA n/a
4 TRCN0000293703 GTCCTGCCCAGTGGGTATTAA pLKO_005 675 CDS 100% 15.000 10.500 N MSRA n/a
5 TRCN0000046456 CCAGCCAGAACACATGAGTTT pLKO.1 363 CDS 100% 4.950 3.465 N MSRA n/a
6 TRCN0000046457 TGGGTCTTGAAAGGAGTGTAT pLKO.1 241 CDS 100% 4.950 3.465 N MSRA n/a
7 TRCN0000286327 TGGGTCTTGAAAGGAGTGTAT pLKO_005 241 CDS 100% 4.950 3.465 N MSRA n/a
8 TRCN0000046454 GCAGGAGGCTATACTTCAAAT pLKO.1 280 CDS 100% 13.200 7.920 N MSRA n/a
9 TRCN0000298176 GCAGGAGGCTATACTTCAAAT pLKO_005 280 CDS 100% 13.200 7.920 N MSRA n/a
10 TRCN0000042095 CTGGGTCTTGAAAGGAGTGTA pLKO.1 240 CDS 100% 4.950 2.970 N Msra n/a
11 TRCN0000334723 CTGGGTCTTGAAAGGAGTGTA pLKO_005 240 CDS 100% 4.950 2.970 N Msra n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017013450.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01038 pDONR223 100% 71.9% 71.9% None 0_1ins198 n/a
2 ccsbBroad304_01038 pLX_304 0% 71.9% 71.9% V5 0_1ins198 n/a
3 TRCN0000480533 TGCGCTCTCTAGAGGGTAGACACG pLX_317 51.7% 71.9% 71.9% V5 0_1ins198 n/a
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