Transcript: Human XM_017013714.2

PREDICTED: Homo sapiens tRNA methyltransferase 9B (putative) (TRMT9B), transcript variant X19, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TRMT9B (57604)
Length:
9558
CDS:
722..1825

Additional Resources:

NCBI RefSeq record:
XM_017013714.2
NBCI Gene record:
TRMT9B (57604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017013714.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140695 GCTATGAACCTGCTATGGCAA pLKO.1 1107 CDS 100% 2.640 3.696 N TRMT9B n/a
2 TRCN0000143010 CCAGGCACTCTGAAACATTTA pLKO.1 1439 CDS 100% 13.200 9.240 N TRMT9B n/a
3 TRCN0000127883 GAGACTCACACAAGAGGTTTA pLKO.1 434 5UTR 100% 10.800 7.560 N TRMT9B n/a
4 TRCN0000144063 CCATAGGAGTCATACATCATT pLKO.1 780 CDS 100% 5.625 3.938 N TRMT9B n/a
5 TRCN0000127859 GAAGGACCGCACTATTTCATT pLKO.1 461 5UTR 100% 5.625 3.938 N TRMT9B n/a
6 TRCN0000143219 GCACAGATTCTGGCATTGAAA pLKO.1 1989 3UTR 100% 5.625 3.938 N TRMT9B n/a
7 TRCN0000143814 GCCAGTAATTCCTCTGTCTTT pLKO.1 5635 3UTR 100% 4.950 3.465 N TRMT9B n/a
8 TRCN0000150057 CAAGAGGTTTATCATGAGAAG pLKO.1 444 5UTR 100% 4.050 2.835 N TRMT9B n/a
9 TRCN0000129167 GCACTATTTCATTTCACTCCT pLKO.1 469 5UTR 100% 2.640 1.848 N TRMT9B n/a
10 TRCN0000144663 GCCTTTCTATTTCCTTCCAAA pLKO.1 5606 3UTR 100% 4.950 2.970 N TRMT9B n/a
11 TRCN0000245346 GGGCTTGTATATAGATTATAA pLKO_005 6197 3UTR 100% 15.000 7.500 Y Prrc2a n/a
12 TRCN0000139826 CCTCCTGAATAGCTGGGATTA pLKO.1 8476 3UTR 100% 10.800 5.400 Y SYNPO2 n/a
13 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 5105 3UTR 100% 13.200 6.600 Y IQCC n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2511 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 5202 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017013714.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12375 pDONR223 100% 99.2% 98.9% None (many diffs) n/a
2 ccsbBroad304_12375 pLX_304 0% 99.2% 98.9% V5 (many diffs) n/a
3 TRCN0000465888 CATAGAGTTGCCTGGTATGAGCGT pLX_317 31.8% 99.2% 98.9% V5 (many diffs) n/a
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