Transcript: Human XM_017018251.1

PREDICTED: Homo sapiens splicing factor 1 (SF1), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SF1 (7536)
Length:
3284
CDS:
502..2076

Additional Resources:

NCBI RefSeq record:
XM_017018251.1
NBCI Gene record:
SF1 (7536)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018251.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218954 GATAAAGCACGGATGGATAAA pLKO_005 1069 CDS 100% 13.200 18.480 N SF1 n/a
2 TRCN0000257126 GGTTGCACTCAATCCGGATTT pLKO_005 504 CDS 100% 10.800 15.120 N SF1 n/a
3 TRCN0000001073 AGGATAAAGCACGGATGGATA pLKO.1 1067 CDS 100% 4.950 6.930 N SF1 n/a
4 TRCN0000001071 CGTAGCATCGAGTGTCTTCTT pLKO.1 2298 3UTR 100% 4.950 6.930 N SF1 n/a
5 TRCN0000001072 GCTCGCTTAAATGGGACCCTT pLKO.1 898 CDS 100% 2.640 3.696 N SF1 n/a
6 TRCN0000219049 AGAGAATTGGTCTCGTTTAAA pLKO_005 2111 3UTR 100% 15.000 12.000 N SF1 n/a
7 TRCN0000229968 AGAACAAGAAAGAGCTTATAT pLKO_005 370 5UTR 100% 15.000 10.500 N SF1 n/a
8 TRCN0000229969 CGGGAGTTGGCTCGCTTAAAT pLKO_005 889 CDS 100% 15.000 10.500 N SF1 n/a
9 TRCN0000109279 GCACGGATGGATAAAGAATAT pLKO.1 1075 CDS 100% 13.200 9.240 N Sf1 n/a
10 TRCN0000001069 GCGTTCTCTTCACTGGTTTAT pLKO.1 3171 3UTR 100% 13.200 9.240 N SF1 n/a
11 TRCN0000001070 CCACAAGATGAGTACCCAGAA pLKO.1 583 CDS 100% 4.050 2.835 N SF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018251.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01789 pDONR223 100% 66.5% 64.3% None (many diffs) n/a
2 ccsbBroad304_01789 pLX_304 0% 66.5% 64.3% V5 (many diffs) n/a
3 TRCN0000469808 GGGAACTTCGTCACAAGTTGTAGT pLX_317 26.7% 66.5% 64.3% V5 (many diffs) n/a
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