Construct: ORF TRCN0000469808

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016525.1_s317c1
Derived from:
ccsbBroadEn_01789
DNA Barcode:
GGGAACTTCGTCACAAGTTGTAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SF1 (7536)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469808
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7536 SF1 splicing factor 1 NM_201998.3 100% 100%
2 human 7536 SF1 splicing factor 1 NM_201997.2 95.9% 77.9% 1343_1346delGTAA;1649_1713del
3 human 7536 SF1 splicing factor 1 NM_001346364.2 95.9% 78.3% 1341_1342insGATCA;1640_1704del
4 human 7536 SF1 splicing factor 1 XM_011545248.1 91.4% 73.6% 0_1ins78;1265_1268delGTAA;1571_1635del
5 human 7536 SF1 splicing factor 1 XM_017018246.1 91.3% 74% 0_1ins78;1263_1264insGATCA;1562_1626del
6 human 7536 SF1 splicing factor 1 XM_024448679.1 88.3% 73.4% (many diffs)
7 human 7536 SF1 splicing factor 1 NM_001346363.2 88.2% 73.7% (many diffs)
8 human 7536 SF1 splicing factor 1 NM_201995.2 85.8% 82.3% 1583_1787del;1850_1914del
9 human 7536 SF1 splicing factor 1 NM_004630.4 84.4% 82.2% (many diffs)
10 human 7536 SF1 splicing factor 1 XM_011545245.1 84% 69.3% (many diffs)
11 human 7536 SF1 splicing factor 1 XM_011545246.1 83.9% 69.7% (many diffs)
12 human 7536 SF1 splicing factor 1 XM_017018245.1 82.9% 80.8% (many diffs)
13 human 7536 SF1 splicing factor 1 XM_011545247.1 82.9% 68.4% (many diffs)
14 human 7536 SF1 splicing factor 1 XM_011545244.1 81.8% 78.3% 0_1ins78;1505_1709del;1772_1836del
15 human 7536 SF1 splicing factor 1 NM_001178030.1 80.9% 79.7% (many diffs)
16 human 7536 SF1 splicing factor 1 NM_001178031.3 80.4% 78.2% (many diffs)
17 human 7536 SF1 splicing factor 1 XM_017018244.1 80.4% 78.2% (many diffs)
18 human 7536 SF1 splicing factor 1 NM_001346409.2 79% 79% 0_1ins345
19 human 7536 SF1 splicing factor 1 XM_024448680.1 79% 79% 0_1ins345
20 human 7536 SF1 splicing factor 1 NM_001346410.2 75.8% 58.8% 0_1ins345;998_1001delGTAA;1304_1368del
21 human 7536 SF1 splicing factor 1 XM_017018249.2 66.5% 64.3% (many diffs)
22 human 7536 SF1 splicing factor 1 XM_017018250.1 66.5% 64.3% (many diffs)
23 human 7536 SF1 splicing factor 1 XM_017018251.1 66.5% 64.3% (many diffs)
24 mouse 22668 Sf1 splicing factor 1 NM_011750.2 92.6% 99.4% (many diffs)
25 mouse 22668 Sf1 splicing factor 1 XM_006531737.3 88.9% 77.1% (many diffs)
26 mouse 22668 Sf1 splicing factor 1 XM_006531738.3 88.8% 77.6% (many diffs)
27 mouse 22668 Sf1 splicing factor 1 XM_006531734.3 81.6% 72.8% (many diffs)
28 mouse 22668 Sf1 splicing factor 1 XM_006531735.3 81.5% 72.9% (many diffs)
29 mouse 22668 Sf1 splicing factor 1 XM_017318187.1 80.9% 62.5% (many diffs)
30 mouse 22668 Sf1 splicing factor 1 XM_006531740.3 79.5% 81.9% (many diffs)
31 mouse 22668 Sf1 splicing factor 1 NM_001110791.1 78% 81.8% (many diffs)
32 mouse 22668 Sf1 splicing factor 1 XM_006531733.3 77% 80.7% (many diffs)
33 mouse 22668 Sf1 splicing factor 1 XM_006531736.1 71% 67.5% (many diffs)
34 mouse 22668 Sf1 splicing factor 1 NR_122118.1 32.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1710
ORF length:
1644
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gaccggagcg aacgccacgc cgttggactt cccaagtaag aagcggaaga
121ggagccgctg gaaccaagac acaatggaac agaagacagt gattccagga atgcctacag
181ttattccccc tggacttact cgagaacaag aaagagctta tatagtgcaa ctgcagatag
241aagacctgac tcgtaaactg cgcacaggag acctgggcat cccccctaac cctgaggaca
301ggtccccttc ccctgagccc atctacaata gcgaggggaa gcggcttaac acccgagagt
361tccgcacccg caaaaagctg gaagaggagc ggcacaacct catcacagag atggttgcac
421tcaatccgga tttcaagcca cctgcagatt acaaacctcc agcaacacgt gtgagtgata
481aagtcatgat tccacaagat gagtacccag aaatcaactt tgtggggctg ctcatcgggc
541ccagagggaa caccctgaag aacatagaga aggagtgcaa tgccaagatt atgatccggg
601ggaaagggtc tgtgaaagaa gggaaggttg ggcgcaaaga tggccagatg ttgccaggag
661aagatgagcc acttcatgcc ctggttactg ccaatacaat ggagaacgtc aaaaaggcag
721tggaacagat aagaaacatc ctgaagcagg gtatcgagac tccagaggac cagaatgatc
781tacggaagat gcagcttcgg gagttggctc gcttaaatgg gacccttcgg gaagacgata
841acaggatctt aagaccctgg cagagctcag agacccgcag cattaccaac accacagtgt
901gtaccaagtg tggaggggct ggccacattg cttcagactg taaattccaa aggcctggtg
961atcctcagtc agctcaggat aaagcacgga tggataaaga atatttgtcc ctcatggctg
1021aactgggtga agcacctgtc ccagcatctg tgggctccac ctctgggcct gccaccacac
1081ccctggccag cgcacctcgt cctgctgctc ccgccaacaa cccacctcca ccgtctctca
1141tgtctaccac ccagagccgc ccaccctgga tgaattctgg cccttcagag agtcggccct
1201accacggcat gcatggaggt ggtcctggtg ggcccggagg tggcccccac agcttcccac
1261acccattacc cagccTGACA GGTGGGCATG GTGGACATCC CATGCAGCAC AACCCCAATG
1321GACCCCCACC CCCTTGGATG CAGCCACCAC CACCACCGAT GAACCAGGGC CCCCACCCTC
1381CTGGGCACCA TGGCCCTCCT CCAATGGATC AGTACCTGGG AAGTACGCCT GTGGGCTCTG
1441GGGTCTATCG CCTGCATCAA GGAAAAGGTA TGATGCCGCC ACCACCTATG GGCATGATGC
1501CGCCGCCGCC GCCGCCTCCC AGTGGGCAGC CCCCACCCCC TCCCTCTGGT CCTCTTCCCC
1561CATGGCAACA ACAGCAGCAG CAGCCTCCGC CACCCCCTCC GCCCAGCAGC AGTATGGCTT
1621CCAGTACCCC CTTGCCATGG CAGCAAAGAT CCCTCCCCGC GGCGGCGATG GCCCGAGCCA
1681TGAGAGTGAG GACTTTCCGC GCCCATTGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1741TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1801CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGAA
1861CTTCGTCACA AGTTGTAGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1921tgaaagatt

Download FASTA (ORF) (Full)