Construct: ORF TRCN0000469808
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016525.1_s317c1
- Derived from:
- ccsbBroadEn_01789
- DNA Barcode:
- GGGAACTTCGTCACAAGTTGTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SF1 (7536)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469808
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7536 | SF1 | splicing factor 1 | NM_201998.3 | 100% | 100% | |
| 2 | human | 7536 | SF1 | splicing factor 1 | NM_201997.2 | 95.9% | 77.9% | 1343_1346delGTAA;1649_1713del |
| 3 | human | 7536 | SF1 | splicing factor 1 | NM_001346364.2 | 95.9% | 78.3% | 1341_1342insGATCA;1640_1704del |
| 4 | human | 7536 | SF1 | splicing factor 1 | XM_011545248.1 | 91.4% | 73.6% | 0_1ins78;1265_1268delGTAA;1571_1635del |
| 5 | human | 7536 | SF1 | splicing factor 1 | XM_017018246.1 | 91.3% | 74% | 0_1ins78;1263_1264insGATCA;1562_1626del |
| 6 | human | 7536 | SF1 | splicing factor 1 | XM_024448679.1 | 88.3% | 73.4% | (many diffs) |
| 7 | human | 7536 | SF1 | splicing factor 1 | NM_001346363.2 | 88.2% | 73.7% | (many diffs) |
| 8 | human | 7536 | SF1 | splicing factor 1 | NM_201995.2 | 85.8% | 82.3% | 1583_1787del;1850_1914del |
| 9 | human | 7536 | SF1 | splicing factor 1 | NM_004630.4 | 84.4% | 82.2% | (many diffs) |
| 10 | human | 7536 | SF1 | splicing factor 1 | XM_011545245.1 | 84% | 69.3% | (many diffs) |
| 11 | human | 7536 | SF1 | splicing factor 1 | XM_011545246.1 | 83.9% | 69.7% | (many diffs) |
| 12 | human | 7536 | SF1 | splicing factor 1 | XM_017018245.1 | 82.9% | 80.8% | (many diffs) |
| 13 | human | 7536 | SF1 | splicing factor 1 | XM_011545247.1 | 82.9% | 68.4% | (many diffs) |
| 14 | human | 7536 | SF1 | splicing factor 1 | XM_011545244.1 | 81.8% | 78.3% | 0_1ins78;1505_1709del;1772_1836del |
| 15 | human | 7536 | SF1 | splicing factor 1 | NM_001178030.1 | 80.9% | 79.7% | (many diffs) |
| 16 | human | 7536 | SF1 | splicing factor 1 | NM_001178031.3 | 80.4% | 78.2% | (many diffs) |
| 17 | human | 7536 | SF1 | splicing factor 1 | XM_017018244.1 | 80.4% | 78.2% | (many diffs) |
| 18 | human | 7536 | SF1 | splicing factor 1 | NM_001346409.2 | 79% | 79% | 0_1ins345 |
| 19 | human | 7536 | SF1 | splicing factor 1 | XM_024448680.1 | 79% | 79% | 0_1ins345 |
| 20 | human | 7536 | SF1 | splicing factor 1 | NM_001346410.2 | 75.8% | 58.8% | 0_1ins345;998_1001delGTAA;1304_1368del |
| 21 | human | 7536 | SF1 | splicing factor 1 | XM_017018249.2 | 66.5% | 64.3% | (many diffs) |
| 22 | human | 7536 | SF1 | splicing factor 1 | XM_017018250.1 | 66.5% | 64.3% | (many diffs) |
| 23 | human | 7536 | SF1 | splicing factor 1 | XM_017018251.1 | 66.5% | 64.3% | (many diffs) |
| 24 | mouse | 22668 | Sf1 | splicing factor 1 | NM_011750.2 | 92.6% | 99.4% | (many diffs) |
| 25 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531737.3 | 88.9% | 77.1% | (many diffs) |
| 26 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531738.3 | 88.8% | 77.6% | (many diffs) |
| 27 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531734.3 | 81.6% | 72.8% | (many diffs) |
| 28 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531735.3 | 81.5% | 72.9% | (many diffs) |
| 29 | mouse | 22668 | Sf1 | splicing factor 1 | XM_017318187.1 | 80.9% | 62.5% | (many diffs) |
| 30 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531740.3 | 79.5% | 81.9% | (many diffs) |
| 31 | mouse | 22668 | Sf1 | splicing factor 1 | NM_001110791.1 | 78% | 81.8% | (many diffs) |
| 32 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531733.3 | 77% | 80.7% | (many diffs) |
| 33 | mouse | 22668 | Sf1 | splicing factor 1 | XM_006531736.1 | 71% | 67.5% | (many diffs) |
| 34 | mouse | 22668 | Sf1 | splicing factor 1 | NR_122118.1 | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1710
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccggagcg aacgccacgc cgttggactt cccaagtaag aagcggaaga 121 ggagccgctg gaaccaagac acaatggaac agaagacagt gattccagga atgcctacag 181 ttattccccc tggacttact cgagaacaag aaagagctta tatagtgcaa ctgcagatag 241 aagacctgac tcgtaaactg cgcacaggag acctgggcat cccccctaac cctgaggaca 301 ggtccccttc ccctgagccc atctacaata gcgaggggaa gcggcttaac acccgagagt 361 tccgcacccg caaaaagctg gaagaggagc ggcacaacct catcacagag atggttgcac 421 tcaatccgga tttcaagcca cctgcagatt acaaacctcc agcaacacgt gtgagtgata 481 aagtcatgat tccacaagat gagtacccag aaatcaactt tgtggggctg ctcatcgggc 541 ccagagggaa caccctgaag aacatagaga aggagtgcaa tgccaagatt atgatccggg 601 ggaaagggtc tgtgaaagaa gggaaggttg ggcgcaaaga tggccagatg ttgccaggag 661 aagatgagcc acttcatgcc ctggttactg ccaatacaat ggagaacgtc aaaaaggcag 721 tggaacagat aagaaacatc ctgaagcagg gtatcgagac tccagaggac cagaatgatc 781 tacggaagat gcagcttcgg gagttggctc gcttaaatgg gacccttcgg gaagacgata 841 acaggatctt aagaccctgg cagagctcag agacccgcag cattaccaac accacagtgt 901 gtaccaagtg tggaggggct ggccacattg cttcagactg taaattccaa aggcctggtg 961 atcctcagtc agctcaggat aaagcacgga tggataaaga atatttgtcc ctcatggctg 1021 aactgggtga agcacctgtc ccagcatctg tgggctccac ctctgggcct gccaccacac 1081 ccctggccag cgcacctcgt cctgctgctc ccgccaacaa cccacctcca ccgtctctca 1141 tgtctaccac ccagagccgc ccaccctgga tgaattctgg cccttcagag agtcggccct 1201 accacggcat gcatggaggt ggtcctggtg ggcccggagg tggcccccac agcttcccac 1261 acccattacc cagccTGACA GGTGGGCATG GTGGACATCC CATGCAGCAC AACCCCAATG 1321 GACCCCCACC CCCTTGGATG CAGCCACCAC CACCACCGAT GAACCAGGGC CCCCACCCTC 1381 CTGGGCACCA TGGCCCTCCT CCAATGGATC AGTACCTGGG AAGTACGCCT GTGGGCTCTG 1441 GGGTCTATCG CCTGCATCAA GGAAAAGGTA TGATGCCGCC ACCACCTATG GGCATGATGC 1501 CGCCGCCGCC GCCGCCTCCC AGTGGGCAGC CCCCACCCCC TCCCTCTGGT CCTCTTCCCC 1561 CATGGCAACA ACAGCAGCAG CAGCCTCCGC CACCCCCTCC GCCCAGCAGC AGTATGGCTT 1621 CCAGTACCCC CTTGCCATGG CAGCAAAGAT CCCTCCCCGC GGCGGCGATG GCCCGAGCCA 1681 TGAGAGTGAG GACTTTCCGC GCCCATTGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1741 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1801 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGAA 1861 CTTCGTCACA AGTTGTAGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1921 tgaaagatt