Transcript: Human XM_017019380.1

PREDICTED: Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DHH (50846)
Length:
4632
CDS:
380..1429

Additional Resources:

NCBI RefSeq record:
XM_017019380.1
NBCI Gene record:
DHH (50846)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017019380.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033315 CGTGTCGGTCAAAGCTGATAA pLKO.1 787 CDS 100% 13.200 18.480 N DHH n/a
2 TRCN0000033316 CGCTCAGGATTCACTCCACTA pLKO.1 643 CDS 100% 4.050 5.670 N DHH n/a
3 TRCN0000033317 GCGTTGTAAGGAGCGGGTGAA pLKO.1 541 CDS 100% 1.350 1.890 N DHH n/a
4 TRCN0000372038 CGTACTGAGAGGAGGTGATAC pLKO_005 1582 3UTR 100% 10.800 7.560 N DHH n/a
5 TRCN0000377821 TGAGCGTCCCAGGCATAGAAA pLKO_005 1427 CDS 100% 5.625 3.938 N DHH n/a
6 TRCN0000033318 GCCGTGCTTTGGACATCACTA pLKO.1 669 CDS 100% 4.950 3.465 N DHH n/a
7 TRCN0000031072 CAGGATTCACTCCACTACGAA pLKO.1 647 CDS 100% 3.000 2.100 N Dhh n/a
8 TRCN0000372096 CTCCTTAAAGAGGACTATTTA pLKO_005 1636 3UTR 100% 15.000 9.000 N DHH n/a
9 TRCN0000073773 CCTCTCAAGTAGCTGGGACTA pLKO.1 3687 3UTR 100% 4.050 2.025 Y TINAGL1 n/a
10 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 3692 3UTR 100% 2.640 1.320 Y LINC01098 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017019380.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03155 pDONR223 100% 82.8% 77% None (many diffs) n/a
2 ccsbBroad304_03155 pLX_304 0% 82.8% 77% V5 (many diffs) n/a
3 TRCN0000476757 TGCTCGCTCCCCGGGTTGCCTTGT pLX_317 31.8% 82.8% 77% V5 (many diffs) n/a
Download CSV