Construct: ORF TRCN0000476757

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013279.1_s317c1
Derived from:
ccsbBroadEn_03155
DNA Barcode:
TGCTCGCTCCCCGGGTTGCCTTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHH (50846)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476757
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50846 DHH desert hedgehog signaling m... NM_021044.4 100% 100%
2 human 50846 DHH desert hedgehog signaling m... XM_017019380.1 82.8% 77% (many diffs)
3 human 50846 DHH desert hedgehog signaling m... XM_017019381.1 71.2% 71.2% 0_1ins342
4 mouse 13363 Dhh desert hedgehog NM_007857.5 88.3% 96.4% (many diffs)
5 mouse 13363 Dhh desert hedgehog XR_875235.2 63.5% (many diffs)
6 mouse 13363 Dhh desert hedgehog XM_006520406.3 63.2% 69.6% (many diffs)
7 mouse 13363 Dhh desert hedgehog XM_017316427.1 63.2% 69.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1254
ORF length:
1188
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tctcctgacc aatctactgc ccctgtgctg cttggcactt ctggcgctgc
121cagcccagag ctgcgggccg ggccgggggc cggttggccg gcgccgctat gcgcgcaagc
181agctcgtgcc gctactctac aagcaatttg tgcccggcgt gccagagcgg accctgggcg
241ccagtgggcc agcggagggg agggtggcaa ggggctccga gcgcttccgg gacctcgtgc
301ccaactacaa ccccgacatc atcttcaagg atgaggagaa cagtggagcc gaccgcctga
361tgaccgagcg ttgtaaggag cgggtgaacg ctttggccat tgccgtgatg aacatgtggc
421ccggagtgcg cctacgagtg actgagggct gggacgagga cggccaccac gctcaggatt
481cactccacta cgaaggccgt gctttggaca tcactacgtc tgaccgcgac cgcaacaagt
541atgggttgct ggcgcgcctc gcagtggaag ccggcttcga ctgggtctac tacgagtccc
601gcaaccacgt ccacgtgtcg gtcaaagctg ataactcact ggcggtccgg gcgggcggct
661gctttccggg aaatgcaact gtgcgcctgt ggagcggcga gcggaaaggg ctgcgggaac
721tgcaccgcgg agactgggtt ttggcggccg atgcgtcagg ccgggtggtg cccacgccgg
781tgctgctctt cctggaccgg gacttgcagc gccgggcttc atttgtggct gtggagaccg
841agtggcctcc acgcaaactg ttgctcacgc cctggcaccT GGTGTTTGCC GCTCGAGGGC
901CGGCGCCCGC GCCAGGCGAC TTTGCACCGG TGTTCGCGCG CCGGCTACGC GCTGGGGACT
961CGGTGCTGGC GCCCGGCGGG GATGCGCTTC GGCCAGCGCG CGTGGCCCGT GTGGCGCGGG
1021AGGAAGCCGT GGGCGTGTTC GCGCCGCTCA CCGCGCACGG GACGCTGCTG GTGAACGATG
1081TCCTGGCCTC TTGCTACGCG GTTCTGGAGA GTCACCAGTG GGCGCACCGC GCTTTTGCCC
1141CCTTGAGACT GCTGCACGCG CTAGGGGCGC TGCTCCCCGG CGGGGCCGTC CAGCCGACTG
1201GCATGCATTG GTACTCTCGG CTCCTCTACC GCTTAGCGGA GGAGCTACTG GGCTGCCCAA
1261CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1321ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1381TATCTTGTGG AAAGGACGAT GCTCGCTCCC CGGGTTGCCT TGTACGCGTT AAGTCgacaa
1441tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)