Transcript: Human XM_017021631.1

PREDICTED: Homo sapiens zinc finger and BTB domain containing 25 (ZBTB25), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZBTB25 (7597)
Length:
2199
CDS:
303..1676

Additional Resources:

NCBI RefSeq record:
XM_017021631.1
NBCI Gene record:
ZBTB25 (7597)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021631.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000021295 CGCTTGTCACAAGAACACTTA pLKO.1 1590 CDS 100% 4.950 6.930 N ZBTB25 n/a
2 TRCN0000021294 GCCGACTACCTTTCTCACATT pLKO.1 678 CDS 100% 4.950 6.930 N ZBTB25 n/a
3 TRCN0000275885 GCCGACTACCTTTCTCACATT pLKO_005 678 CDS 100% 4.950 6.930 N ZBTB25 n/a
4 TRCN0000021298 CCAACCTGACATATTCAGCTA pLKO.1 572 CDS 100% 2.640 3.696 N ZBTB25 n/a
5 TRCN0000285454 CCAACCTGACATATTCAGCTA pLKO_005 572 CDS 100% 2.640 3.696 N ZBTB25 n/a
6 TRCN0000021296 GCCAGTAGAATTAAACTGTAA pLKO.1 1367 CDS 100% 4.950 3.960 N ZBTB25 n/a
7 TRCN0000021297 GCTTCCATTCTGGAAAGTAAT pLKO.1 1179 CDS 100% 13.200 9.240 N ZBTB25 n/a
8 TRCN0000275884 GCTTCCATTCTGGAAAGTAAT pLKO_005 1179 CDS 100% 13.200 9.240 N ZBTB25 n/a
9 TRCN0000275886 CAGATCAGTCAAGTATCTTTG pLKO_005 1329 CDS 100% 10.800 7.560 N ZBTB25 n/a
10 TRCN0000275946 TTGGAGGAAGGGATTCGATTT pLKO_005 651 CDS 100% 10.800 6.480 N ZBTB25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021631.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01803 pDONR223 100% 95.1% 95.1% None 1_66del n/a
2 ccsbBroad304_01803 pLX_304 0% 95.1% 95.1% V5 1_66del n/a
3 TRCN0000481016 CAGCGCAGAGCACTAAGCAGGTTG pLX_317 31.6% 95.1% 95.1% V5 1_66del n/a
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