Transcript: Human XM_017021736.1

PREDICTED: Homo sapiens DDB1 and CUL4 associated factor 5 (DCAF5), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DCAF5 (8816)
Length:
5638
CDS:
125..2704

Additional Resources:

NCBI RefSeq record:
XM_017021736.1
NBCI Gene record:
DCAF5 (8816)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021736.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425851 TGTTAACCAAGTCCGATTTAA pLKO_005 880 CDS 100% 15.000 21.000 N DCAF5 n/a
2 TRCN0000146480 CCCAACTTTGATGGCACATTT pLKO.1 5238 3UTR 100% 13.200 18.480 N DCAF5 n/a
3 TRCN0000127609 CCGCTACTCTTATATCTCCTA pLKO.1 1876 CDS 100% 2.640 3.696 N DCAF5 n/a
4 TRCN0000424290 AGGAGATCGTGACCAGTATAT pLKO_005 739 CDS 100% 13.200 17.160 N DCAF5 n/a
5 TRCN0000150046 CGGATACATATCTTCCTGAAA pLKO.1 4830 3UTR 100% 4.950 3.960 N DCAF5 n/a
6 TRCN0000147371 GCCAGTGAGTTGTAGATTTAT pLKO.1 3187 3UTR 100% 15.000 10.500 N DCAF5 n/a
7 TRCN0000429635 AGATTGTTGCGGGTCTGAAAT pLKO_005 2518 CDS 100% 13.200 9.240 N DCAF5 n/a
8 TRCN0000436757 ATGGCACGTGTGCGCTTATTG pLKO_005 3034 3UTR 100% 13.200 9.240 N DCAF5 n/a
9 TRCN0000130185 CCAACTTTGATGGCACATTTA pLKO.1 5239 3UTR 100% 13.200 9.240 N DCAF5 n/a
10 TRCN0000439523 CTCAGACACTGACCGTGATAA pLKO_005 2467 CDS 100% 13.200 9.240 N DCAF5 n/a
11 TRCN0000147444 GCCTTTCATAGTGTCATGTTT pLKO.1 440 CDS 100% 5.625 3.938 N DCAF5 n/a
12 TRCN0000127709 GCCTGTGTTTCAGTTTGACAA pLKO.1 673 CDS 100% 4.950 3.465 N DCAF5 n/a
13 TRCN0000127994 GCATAGTGAAAGAAGGGCAAA pLKO.1 3584 3UTR 100% 4.050 2.835 N DCAF5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021736.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11308 pDONR223 100% 24.2% 23% None (many diffs) n/a
2 ccsbBroad304_11308 pLX_304 0% 24.2% 23% V5 (many diffs) n/a
3 TRCN0000469590 ATCGCCAGACCCGGACCTCCCGAG pLX_317 44.9% 24.2% 23% V5 (many diffs) n/a
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