Transcript: Human XM_017028108.2

PREDICTED: Homo sapiens ribonucleic acid export 1 (RAE1), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RAE1 (8480)
Length:
1744
CDS:
192..1265

Additional Resources:

NCBI RefSeq record:
XM_017028108.2
NBCI Gene record:
RAE1 (8480)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017028108.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000337192 TGGGATACTCGATCGTCAAAT pLKO_005 657 CDS 100% 13.200 18.480 N RAE1 n/a
2 TRCN0000160979 GAGGATAGAATCTCCACTGAA pLKO.1 806 CDS 100% 4.950 6.930 N RAE1 n/a
3 TRCN0000158987 GAAACATATTTGCATACGCTT pLKO.1 1171 CDS 100% 2.640 3.696 N RAE1 n/a
4 TRCN0000164681 CCTCAGGACATTTATGCGGTA pLKO.1 999 CDS 100% 2.160 3.024 N RAE1 n/a
5 TRCN0000305592 ATCACAATGGAAACATATTTG pLKO_005 1162 CDS 100% 13.200 9.240 N Rae1 n/a
6 TRCN0000158931 GCTTGCTGTTTCAATCACAAT pLKO.1 1149 CDS 100% 4.950 3.465 N RAE1 n/a
7 TRCN0000337123 GCTTGCTGTTTCAATCACAAT pLKO_005 1149 CDS 100% 4.950 3.465 N RAE1 n/a
8 TRCN0000165752 CCAGAGTTGTTTCTCTCCACT pLKO.1 1408 3UTR 100% 2.640 1.848 N RAE1 n/a
9 TRCN0000279721 CCAGAGTTGTTTCTCTCCACT pLKO_005 1408 3UTR 100% 2.640 1.848 N RAE1 n/a
10 TRCN0000166724 CGATGTCATTGTTCAGCAGCT pLKO.1 1510 3UTR 100% 2.160 1.512 N RAE1 n/a
11 TRCN0000279669 CGATGTCATTGTTCAGCAGCT pLKO_005 1510 3UTR 100% 2.160 1.512 N RAE1 n/a
12 TRCN0000162728 CCATTGGATCAAAGCTCCAAA pLKO.1 590 CDS 100% 0.495 0.347 N RAE1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017028108.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01936 pDONR223 100% 94.4% 90.2% None (many diffs) n/a
2 ccsbBroad304_01936 pLX_304 0% 94.4% 90.2% V5 (many diffs) n/a
3 TRCN0000471793 GTGCATGTCAGGCACGTTTTCAGA pLX_317 29.2% 94.4% 90.2% V5 (many diffs) n/a
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