Transcript: Mouse XM_017313024.1

PREDICTED: Mus musculus tumor suppressor candidate 3 (Tusc3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tusc3 (80286)
Length:
2410
CDS:
594..1550

Additional Resources:

NCBI RefSeq record:
XM_017313024.1
NBCI Gene record:
Tusc3 (80286)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017313024.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042443 CGGATGTTCATATTCGAGTTT pLKO.1 1150 CDS 100% 4.950 6.930 N Tusc3 n/a
2 TRCN0000324669 CGGATGTTCATATTCGAGTTT pLKO_005 1150 CDS 100% 4.950 6.930 N Tusc3 n/a
3 TRCN0000042445 CCTGGCGTTATTCATCTGCTT pLKO.1 937 CDS 100% 2.640 2.112 N Tusc3 n/a
4 TRCN0000324735 CCTGGCGTTATTCATCTGCTT pLKO_005 937 CDS 100% 2.640 2.112 N Tusc3 n/a
5 TRCN0000042446 GCAGAGTCACACATCATTCTA pLKO.1 1338 CDS 100% 5.625 3.938 N Tusc3 n/a
6 TRCN0000324668 GCAGAGTCACACATCATTCTA pLKO_005 1338 CDS 100% 5.625 3.938 N Tusc3 n/a
7 TRCN0000042444 GCTTTATCTAAGGAGGAACAA pLKO.1 1235 CDS 100% 4.950 3.465 N Tusc3 n/a
8 TRCN0000324734 GCTTTATCTAAGGAGGAACAA pLKO_005 1235 CDS 100% 4.950 3.465 N Tusc3 n/a
9 TRCN0000042447 CTTCAGAATGAACGGCGACAA pLKO.1 788 CDS 100% 4.050 2.835 N Tusc3 n/a
10 TRCN0000324733 CTTCAGAATGAACGGCGACAA pLKO_005 788 CDS 100% 4.050 2.835 N Tusc3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017313024.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01853 pDONR223 100% 81.1% 89% None (many diffs) n/a
2 ccsbBroad304_01853 pLX_304 0% 81.1% 89% V5 (many diffs) n/a
3 TRCN0000471232 TAGAAGACATCCAAGCTTTATAAA pLX_317 19.5% 81.1% 89% V5 (many diffs) n/a
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