Transcript: Mouse XM_017315842.1

PREDICTED: Mus musculus myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Myh7 (140781)
Length:
6187
CDS:
271..6078

Additional Resources:

NCBI RefSeq record:
XM_017315842.1
NBCI Gene record:
Myh7 (140781)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017315842.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000055315 GCCCGATGACAAAGAAGAGTT pLKO.1 387 CDS 100% 4.950 6.930 N Myh7 n/a
2 TRCN0000055314 GCTCTCCAGAATGGAGTTCAA pLKO.1 2655 CDS 100% 4.950 3.465 N Myh7 n/a
3 TRCN0000055317 GCTCAGCAATCTATTTGCCAA pLKO.1 2118 CDS 100% 2.640 1.848 N Myh7 n/a
4 TRCN0000055313 CCAGAAGAGAAGAACTCCATT pLKO.1 1297 CDS 100% 4.950 2.970 N Myh7 n/a
5 TRCN0000446189 CACCTGGGCAAGTCCAACAAT pLKO_005 1936 CDS 100% 5.625 3.375 N MYH6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017315842.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15503 pDONR223 0% 91.9% 97.5% None (many diffs) n/a
2 ccsbBroad304_15503 pLX_304 0% 91.9% 97.5% V5 (many diffs) n/a
3 TRCN0000466427 GTATGGACTATCGCGATATGCGCC pLX_317 6.9% 91.9% 97.5% V5 (many diffs) n/a
4 TRCN0000470090 CGCTCATCACTAGCCCGGCCCGTT pLX_317 6.9% 91.7% 97.2% V5 (many diffs) n/a
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