Construct: ORF ccsbBroad304_15503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012748.1_s304c1, BRDN0000401876
- Derived from:
- ccsbBroadEn_15503
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYH7 (4625)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15503
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4625 | MYH7 | myosin heavy chain 7 | NM_000257.4 | 100% | 100% | |
| 2 | human | 4625 | MYH7 | myosin heavy chain 7 | XM_017021340.1 | 100% | 100% | |
| 3 | human | 4624 | MYH6 | myosin heavy chain 6 | NM_002471.3 | 90.4% | 92.6% | (many diffs) |
| 4 | mouse | 140781 | Myh7 | myosin, heavy polypeptide 7... | NM_080728.2 | 91.9% | 97.5% | (many diffs) |
| 5 | mouse | 140781 | Myh7 | myosin, heavy polypeptide 7... | XM_017315841.1 | 91.9% | 97.5% | (many diffs) |
| 6 | mouse | 140781 | Myh7 | myosin, heavy polypeptide 7... | XM_017315842.1 | 91.9% | 97.5% | (many diffs) |
| 7 | mouse | 17888 | Myh6 | myosin, heavy polypeptide 6... | NM_001164171.1 | 87.6% | 92% | (many diffs) |
| 8 | mouse | 17888 | Myh6 | myosin, heavy polypeptide 6... | NM_010856.4 | 87.6% | 92% | (many diffs) |
| 9 | mouse | 17888 | Myh6 | myosin, heavy polypeptide 6... | XM_006518677.1 | 87.6% | 91.9% | (many diffs) |
| 10 | mouse | 17888 | Myh6 | myosin, heavy polypeptide 6... | XM_006518678.3 | 87.6% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5874
- ORF length:
- 5805
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagattcg gagatggcag tctttggggc tgccgccccc tacctgcgca 121 agtcagagaa ggagcggcta gaagcgcaga ccaggccttt tgacctcaag aaggatgtct 181 tcgtgcctga tgacaaacag gagtttgtca aggccaagat cgtgtctcga gagggtggca 241 aagtcactgc cgagaccgag tatggcaaga cagtgaccgt gaaggaggac caggtgatgc 301 agcagaaccc acccaagttc gacaaaatcg aggacatggc catgctgacc ttcctgcatg 361 agcccgcggt gctctacaac ctcaaggatc gctacggctc ctggatgatc tacacctact 421 cgggcctctt ctgtgtcacc gtcaaccctt acaagtggct gccggtgtac actcctgagg 481 tggtggctgc ctaccggggc aagaagagga gcgaggcccc gccccacatc ttctccatct 541 ccgacaacgc ctatcagtac atgctgacag acagagaaaa ccagtccatc ctgatcaccg 601 gagaatccgg agcagggaag acagtcaaca ccaagagggt catccagtac tttgctgtta 661 ttgcagccat tggggaccgc agcaagaagg accagagccc gggcaagggc accctggagg 721 accagatcat ccaggccaac cctgctctgg aggcctttgg caatgccaag accgtccgga 781 acgacaactc ctcccgcttc gggaaattca ttcgaattca ttttggggca acaggaaagt 841 tggcatctgc agacatagag acctatcttc tggaaaaatc cagagttatt ttccagctga 901 aagcagagag agattatcac attttctacc aaatcctgtc taacaaaaag cctgagctgc 961 tggacatgct gctgatcacc aacaacccct acgattatgc attcatctcc caaggagaga 1021 ccaccgtggc ctccattgat gacgctgagg agctcatggc cactgataac gcttttgatg 1081 tgctgggctt cacttcagag gagaaaaact ccatgtataa gctgacaggc gccatcatgc 1141 actttggaaa catgaagttc aagctgaagc agcgggagga gcaggcggag ccagacggca 1201 ctgaagaggc tgacaagtct gcctacctca tggggctgaa ctcagccgac ctgctcaagg 1261 ggctgtgcca ccctcgggtg aaagtgggca atgagtacgt caccaagggg cagaatgtcc 1321 agcaggtgat atatgccact ggggcactgg ccaaggcagt gtatgagagg atgttcaact 1381 ggatggtgac gcgcatcaat gccaccctgg agaccaagca gccacgccag tacttcatag 1441 gagtcctgga catcgctggc ttcgagatct tcgatttcaa cagctttgag cagctctgca 1501 tcaacttcac caacgagaag ctgcagcagt tcttcaacca ccacatgttt gtgctggagc 1561 aggaggagta caagaaggag ggcatcgagt ggacattcat tgactttggc atggacctgc 1621 aggcctgcat tgacctcatc gagaagccca tgggcatcat gtccatcctg gaagaggagt 1681 gcatgttccc caaggccacc gacatgacct tcaaggccaa gctgtttgac aaccacctgg 1741 gcaaatccgc caacttccag aagccacgca atatcaaggg gaagcctgaa gcccacttct 1801 ccctgatcca ctatgccggc atcgtggact acaacatcat tggctggctg cagaagaaca 1861 aggatcctct caatgagact gtcgtgggct tgtatcagaa gtcttccctc aagctgctca 1921 gcaccctgtt tgccaactat gctggggctg atgcgcctat tgagaagggc aaaggcaagg 1981 ccaagaaagg ctcgtccttt cagactgtgt cagctctgca cagggaaaat ctgaacaagc 2041 tgatgaccaa cttgcgctcc acccatcccc actttgtacg ttgtatcatc cctaatgaga 2101 caaagtctcc aggggtgatg gacaaccccc tggtcatgca ccagctgcgc tgcaatggtg 2161 tgctggaggg catccgcatc tgcaggaaag gcttccccaa ccgcatcctc tacggggact 2221 tccggcagag gtatcgcatc ctgaacccag cggccatccc tgagggacag ttcattgata 2281 gcaggaaggg ggcagagaag ctgctcagct ccctggacat tgatcacaac cagtacaagt 2341 ttggccacac caaggtgttc ttcaaggccg ggctgctggg gctgctggag gaaatgaggg 2401 acgagaggct gagccgcatc atcacgcgta tccaggccca gtcccgaggt gtgctcgcca 2461 gaatggagta caaaaagctg ctggaacgta gagactccct gctggtaatc cagtggaaca 2521 ttcgggcctt catgggggtc aagaattggc cctggatgaa gctctacttc aagatcaagc 2581 cgctgctgaa gagtgcagaa agagagaagg agatggcctc catgaaggag gagttcacac 2641 gcctcaaaga ggcgctagag aagtccgagg ctcgccgcaa ggagctggag gagaagatgg 2701 tgtccctgct gcaggagaag aatgacctgc agctccaagt gcaggcggaa caagacaacc 2761 tggcagatgc tgaggagcgc tgtgatcagc tgatcaaaaa caagattcag ctggaggcca 2821 aggtgaagga gatgaacgag aggctggagg atgaggagga gatgaatgct gagctcactg 2881 ccaagaagcg caagctggaa gatgagtgct cagagctcaa aagggacatc gatgatctgg 2941 agctgacact ggccaaagtg gagaaggaga aacacgcaac agagaacaag gtgaaaaacc 3001 tgacagagga gatggctggg ctggatgaga tcattgccaa gctgaccaag gagaagaaag 3061 ctctgcaaga ggcccaccaa caggctctgg atgaccttca ggccgaggag gacaaggtca 3121 acaccctgac taaggccaaa gtcaagctgg agcagcaagt ggatgatctg gaaggatccc 3181 tggagcaaga gaagaaggtg cgcatggacc tggagcgagc gaagcggaag ctggagggcg 3241 acctgaagct gacccaggag agcatcatgg acctggagaa tgacaagcag cagctggatg 3301 agcggctgaa aaaaaaagac tttgagctga atgctctcaa cgcaaggatt gaggatgaac 3361 aggccctcgg cagccagctg cagaagaagc tcaaggagct tcaggcacgc atcgaggagc 3421 tggaggagga gctggaggcc gagcgcaccg ccagggctaa ggtggagaag ctgcgctcag 3481 acctgtctcg ggagctggag gagatcagcg agcggctgga agaggccggc ggggccacgt 3541 ccgtgcagat cgagatgaac aagaagcgcg aggccgagtt ccagaagatg cggcgggacc 3601 tggaggaggc cacgctgcag cacgaggcca ctgccgcggc cctgcgcaag aagcacgccg 3661 acagcgtggc cgagctgggc gagcagatcg acaacctgca gcgggtgaag cagaagctgg 3721 agaaggagaa gagcgagttc aagctggagc tggatgacgt cacctccaac atggagcaga 3781 tcatcaaggc caaggctaac ctggagaaga tgtgccggac cttggaagac cagatgaatg 3841 agcaccggag caaggcggag gagacccagc gttctgtcaa cgacctcacc agccagcggg 3901 ccaagttgca aaccgagaat ggtgagctgt cccggcagct ggatgagaag gaggcactga 3961 tctcccagct gacccgaggc aagctcacct acacccagca gctggaggac ctcaagaggc 4021 agctggagga ggaggttaag gcgaagaacg ccctggccca cgcactgcag tcggcccggc 4081 atgactgcga cctgctgcgg gagcagtacg aggaggagac ggaggccaag gccgagctgc 4141 agcgcgtcct ttccaaggcc aactcggagg tggcccagtg gaggaccaag tatgagacgg 4201 acgccattca gcggactgag gagctcgagg aggccaagaa gaagctggcc cagcggctgc 4261 aggaagctga ggaggccgtg gaggctgtta atgccaagtg ctcctcgctg gagaagacca 4321 agcaccggct acagaatgag atcgaggact tgatggtgga cgtagagcgc tccaatgctg 4381 ctgctgcagc cctggacaag aagcagagga acttcgacaa gatcctggcc gagtggaagc 4441 agaagtatga ggagtcgcag tcggagctgg agtcctcgca gaaggaggct cgctccctca 4501 gcacagagct cttcaaactc aagaacgcct atgaggagtc cctggaacat ctggagacct 4561 tcaagcggga gaacaaaaac ctgcaggagg agatctccga cttgactgag cagttgggtt 4621 ccagcggaaa gactatccat gagctggaga aggtccgaaa gcagctggag gccgagaaga 4681 tggagctgca gtcagccctg gaggaggccg aggcctccct ggagcacgag gagggcaaga 4741 tcctccgggc ccagctggag ttcaaccaga tcaaggcaga gatcgagcgg aagctggcag 4801 agaaggacga ggagatggaa caggccaagc gcaaccacct gcgggtggtg gactcgctgc 4861 agacctccct ggacgcagag acacgcagcc gcaacgaggc cctgagggtg aagaagaaga 4921 tggaaggaga cctcaatgag atggagatcc agctcagcca cgccaaccgc atggccgccg 4981 aggcccagaa gcaagtcaag agcctccaga gcttgttgaa ggacacccag attcagctgg 5041 acgatgcagt ccgtgccaac gacgacctga aggagaacat cgccatcgtg gagcggcgca 5101 acaacctgct gcaggctgag ctggaggagt tgcgtgccgt ggtggagcag acagagcggt 5161 cccggaagct ggcggagcag gagctgattg agactagtga gcgggtgcag ctgctgcatt 5221 cccagaacac cagcctcatc aaccagaaga agaagatgga tgctgacctg tcccagctcc 5281 agactgaagt ggaggaggca gtgcaggagt gcaggaatgc tgaggagaag gccaagaagg 5341 ccatcacgga tgccgccatg atggcagagg agctgaagaa ggagcaggac accagcgccc 5401 acctggagcg catgaagaag aacatggaac agaccattaa ggacctgcag caccggctgg 5461 acgaagccga gcagatcgcc ctcaagggcg gcaagaagca gctgcagaag ctggaagcgc 5521 gggtgcggga gctggagaat gagctggagg ccgagcagaa gcgcaacgca gagtcggtga 5581 agggcatgag gaagagcgag cggcgcatca aggagctcac ctaccagacg gaggaggaca 5641 ggaaaaacct gctgcggctg caggacctgg tagacaagct gcagctaaag gtcaaggcct 5701 acaagcgcca ggccgaggag gcggaggagc aagccaacac caacctgtcc aagttccgca 5761 aggtgcagca cgagctggat gaggcagagg agcgggcgga catcgccgag tcccaggtca 5821 acaagctgcg ggccaagagc cgtgacattg gcacgaaggg cttgaatgag gagtnnccaa 5881 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 5941 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta