Transcript: Mouse XM_017317255.1

PREDICTED: Mus musculus bromodomain containing 2 (Brd2), transcript variant X12, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Brd2 (14312)
Length:
2782
CDS:
809..2230

Additional Resources:

NCBI RefSeq record:
XM_017317255.1
NBCI Gene record:
Brd2 (14312)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017317255.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000362394 CCCGGAAGCCCTACACTATTA pLKO_005 1959 CDS 100% 13.200 18.480 N Brd2 n/a
2 TRCN0000362395 CCCTCTCTACGTGATTCAAAT pLKO_005 1847 CDS 100% 13.200 18.480 N Brd2 n/a
3 TRCN0000362476 GATTACCATGACATCATTAAA pLKO_005 811 CDS 100% 15.000 10.500 N Brd2 n/a
4 TRCN0000350530 CTACCACTGTCCTCAACATTC pLKO_005 359 5UTR 100% 10.800 7.560 N BRD2 n/a
5 TRCN0000023962 CAGCCCAAGAAATCTAAGAAA pLKO.1 1574 CDS 100% 5.625 3.938 N Brd2 n/a
6 TRCN0000006312 CCTACCACTGTCCTCAACATT pLKO.1 358 5UTR 100% 5.625 3.938 N BRD2 n/a
7 TRCN0000023961 CCTCAGAATGTATGCAGGATT pLKO.1 71 5UTR 100% 4.950 3.465 N Brd2 n/a
8 TRCN0000023963 GCTTATGTTCTCCAACTGCTA pLKO.1 1087 CDS 100% 2.640 1.848 N Brd2 n/a
9 TRCN0000023959 CCAGAAGAAATTGAGATTGAT pLKO.1 1868 CDS 100% 5.625 3.375 N Brd2 n/a
10 TRCN0000006311 CCTATGGACATGGGTACTATT pLKO.1 16 5UTR 100% 13.200 9.240 N BRD2 n/a
11 TRCN0000273643 CCTATGGACATGGGTACTATT pLKO_005 16 5UTR 100% 13.200 9.240 N BRD2 n/a
12 TRCN0000434193 AGGAGGAGGAAGAAGATGAAG pLKO_005 1314 CDS 100% 4.950 2.475 Y Myt1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017317255.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488280 TTCTCATGCTTAGATCCGTCTCCC pLX_317 12.2% 50.7% 45.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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