Construct: ORF TRCN0000488280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020676.1_s317c1
- DNA Barcode:
- TTCTCATGCTTAGATCCGTCTCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BRD2 (6046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6046 | BRD2 | bromodomain containing 2 | NM_001113182.3 | 99.9% | 100% | 621C>T |
| 2 | human | 6046 | BRD2 | bromodomain containing 2 | NM_005104.4 | 99.9% | 100% | 621C>T |
| 3 | human | 6046 | BRD2 | bromodomain containing 2 | NM_001199455.1 | 95.7% | 95.8% | 621C>T;1842_1946del |
| 4 | human | 6046 | BRD2 | bromodomain containing 2 | NM_001199456.1 | 94% | 94.1% | 0_1ins141;480C>T |
| 5 | human | 6046 | BRD2 | bromodomain containing 2 | NM_001291986.2 | 84.9% | 85% | 0_1ins360;261C>T |
| 6 | human | 6046 | BRD2 | bromodomain containing 2 | XR_001743557.2 | 70.6% | (many diffs) | |
| 7 | human | 6046 | BRD2 | bromodomain containing 2 | XR_241911.3 | 50.1% | (many diffs) | |
| 8 | mouse | 14312 | Brd2 | bromodomain containing 2 | NM_001204973.1 | 89% | 96.3% | (many diffs) |
| 9 | mouse | 14312 | Brd2 | bromodomain containing 2 | NM_010238.3 | 89% | 96.3% | (many diffs) |
| 10 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317245.1 | 82.2% | 88.9% | (many diffs) |
| 11 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317246.1 | 82.2% | 88.9% | (many diffs) |
| 12 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317247.1 | 82.2% | 88.9% | (many diffs) |
| 13 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317248.1 | 82.2% | 88.9% | (many diffs) |
| 14 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317249.1 | 82.2% | 88.9% | (many diffs) |
| 15 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317250.1 | 77.2% | 83.6% | (many diffs) |
| 16 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317253.1 | 75.4% | 81.6% | (many diffs) |
| 17 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317251.1 | 69.6% | 75.3% | (many diffs) |
| 18 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317252.1 | 69.6% | 75.3% | (many diffs) |
| 19 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317255.1 | 50.7% | 45.6% | (many diffs) |
| 20 | mouse | 14312 | Brd2 | bromodomain containing 2 | NR_037970.1 | 49.3% | (many diffs) | |
| 21 | mouse | 14312 | Brd2 | bromodomain containing 2 | XM_017317254.1 | 46.8% | 42.2% | (many diffs) |
| 22 | mouse | 14312 | Brd2 | bromodomain containing 2 | XR_385282.3 | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2475
- ORF length:
- 2403
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctgcaa aacgtgactc cccacaataa gctccctggg gaagggaatg 121 cagggttgct ggggctgggc ccagaagcag cagcaccagg gaagaggatt cgaaaaccct 181 ctctcttgta tgagggcttt gagagcccca caatggcttc ggtgcctgct ttgcaactta 241 cccctgccaa cccaccaccc ccggaggtgt ccaatcccaa aaagccagga cgagttacca 301 accagctgca atacctacac aaggtagtga tgaaggctct gtggaaacat cagttcgcat 361 ggccattccg gcagcctgtg gatgctgtca aactgggtct accggattat cacaaaatta 421 taaaacagcc tatggacatg ggtactatta agaggagact tgaaaacaat tattattggg 481 ctgcttcaga gtgtatgcaa gattttaata ccatgttcac caactgttac atttacaaca 541 agcccactga tgatattgtc ctaatggcac aaacgctgga aaagatattc ctacagaagg 601 ttgcatcaat gccacaagaa gaacaagagc tggtagtgac catccctaag aacagccaca 661 agaagggggc caagttggca gcgctccagg gtagtgttac cagtgcccat caggtgcctg 721 ccgtctcttc tgtgtcacac acagccctgt atactcctcc acctgagata cctaccactg 781 tcctcaacat tccccaccca tcagtcattt cctctccact tctcaagtcc ttgcactctg 841 ctggaccccc gctccttgct gttactgcag ctcctccagc ccagcccctt gccaagaaaa 901 aaggcgtaaa gcggaaagca gatactacca cccctacacc tacagccatc ttggctcctg 961 gttctccagc tagccctcct gggagtcttg agcctaaggc agcacggctt ccccctatgc 1021 gtagagagag tggtcgcccc atcaagcccc cacgcaaaga cttgcctgac tctcagcaac 1081 aacaccagag ctctaagaaa ggaaagcttt cagaacagtt aaaacattgc aatggcattt 1141 tgaaggagtt actctctaag aagcatgctg cctatgcttg gcctttctat aaaccagtgg 1201 atgcttctgc acttggcctg catgactacc atgacatcat taagcacccc atggacctca 1261 gcactgtcaa gcggaagatg gagaaccgtg attaccggga tgcacaggag tttgctgctg 1321 atgtacggct tatgttctcc aactgctata agtacaatcc cccagatcac gatgttgtgg 1381 caatggcacg aaagctacag gatgtatttg agttccgtta tgccaagatg ccagatgaac 1441 cactagaacc agggccttta ccagtctcta ctgccatgcc ccctggcttg gccaaatcgt 1501 cttcagagtc ctccagtgag gaaagtagca gtgagagctc ctctgaggaa gaggaggagg 1561 aagatgagga ggacgaggag gaagaagaga gtgaaagctc agactcagag gaagaaaggg 1621 ctcatcgctt agcagaacta caggaacagc ttcgggcagt acatgaacaa ctggctgctc 1681 tgtcccaggg tccaatatcc aagcccaaga ggaaaagaga gaaaaaagag aaaaagaaga 1741 aacggaaggc agagaagcat cgaggccgag ctggggccga tgaagatgac aaggggccta 1801 gggcaccccg cccacctcaa cctaagaagt ccaagaaagc aagtggcagt gggggtggca 1861 gtgctgcttt aggcccttct ggctttggac cttctggagg aagtggcacc aagctcccca 1921 aaaaggccac aaagacagcc ccacctgccc tgcctacagg ttatgattca gaggaggagg 1981 aagagagcag gcccatgagt tacgatgaga agcggcagct gagcctggac atcaacaaat 2041 tacctgggga gaagctgggc cgagttgtgc atataatcca agccagggag ccctctttac 2101 gtgattcaaa cccagaagag attgagattg attttgaaac actcaagcca tccacactta 2161 gagagcttga gcgctatgtc ctttcctgcc TACGTAAGAA ACCCCGGAAG CCCTACACCA 2221 TTAAGAAGCC TGTGGGAAAG ACAAAGGAGG AACTGGCTTT GGAGAAAAAG CGGGAATTAG 2281 AAAAGCGGTT ACAAGATGTC AGCGGACAGC TCAATTCTAC TAAAAAGCCC CCCAAGAAAG 2341 CGAATGAGAA AACAGAGTCA TCCTCTGCAC AGCAAGTAGC AGTGTCACGC CTTAGCGCTT 2401 CCAGCTCCAG CTCAGATTCC AGCTCCTCCT CTTCCTCGTC GTCGTCTTCA GACACCAGTG 2461 ATTCAGACTC AGGCTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2521 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2581 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCTCAT GCTTAGATCC 2641 GTCTCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt