Transcript: Mouse XM_017317461.1

PREDICTED: Mus musculus phosphodiesterase 10A (Pde10a), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pde10a (23984)
Length:
8078
CDS:
714..3086

Additional Resources:

NCBI RefSeq record:
XM_017317461.1
NBCI Gene record:
Pde10a (23984)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017317461.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000321306 TTCCTACTCGACGTATCAAAG pLKO_005 1479 CDS 100% 10.800 15.120 N Pde10a n/a
2 TRCN0000077318 CCCTTGTTGTGAAGTTTACAT pLKO.1 3466 3UTR 100% 5.625 7.875 N Pde10a n/a
3 TRCN0000321240 GCTTGGGCTTCCGTAGCAATA pLKO_005 1407 CDS 100% 10.800 8.640 N Pde10a n/a
4 TRCN0000005479 CCACTTTGACATTGGTCCTTT pLKO.1 2120 CDS 100% 4.950 3.960 N PDE10A n/a
5 TRCN0000321307 CTTGAACACATCATGATATAT pLKO_005 1536 CDS 100% 15.000 10.500 N Pde10a n/a
6 TRCN0000321308 GAGCGCAAAGGCCTGCTAATT pLKO_005 2349 CDS 100% 13.200 9.240 N Pde10a n/a
7 TRCN0000321305 TGTGACCTTCTTATAGGTTAA pLKO_005 3486 3UTR 100% 10.800 7.560 N Pde10a n/a
8 TRCN0000077320 CGACGGATTTGCACTGTACTT pLKO.1 1052 CDS 100% 4.950 3.465 N Pde10a n/a
9 TRCN0000077321 GCGAATGATATATATGCAGAA pLKO.1 2760 CDS 100% 4.050 2.835 N Pde10a n/a
10 TRCN0000077322 CCTTACCACAACTGGAAGCAT pLKO.1 2262 CDS 100% 3.000 2.100 N Pde10a n/a
11 TRCN0000077319 CGACGTATCAAAGACATACTT pLKO.1 1487 CDS 100% 5.625 3.375 N Pde10a n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 144 5UTR 100% 4.950 2.475 Y KAAG1 n/a
13 TRCN0000178741 CACACACATACACACACACAA pLKO.1 164 5UTR 100% 4.950 2.475 Y Cstad n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017317461.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07690 pDONR223 100% 85% 92.9% None (many diffs) n/a
2 ccsbBroad304_07690 pLX_304 0% 85% 92.9% V5 (many diffs) n/a
3 TRCN0000477378 CGTGACATACTACGCATGCAACGC pLX_317 17.5% 85% 92.9% V5 (many diffs) n/a
4 TRCN0000487706 TACCGCGTATTGAGCTCGGTTCTG pLX_317 10.1% 85% 92.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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