Construct: ORF TRCN0000477378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000066.1_s317c1
- Derived from:
- ccsbBroadEn_07690
- DNA Barcode:
- CGTGACATACTACGCATGCAACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE10A (10846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10846 | PDE10A | phosphodiesterase 10A | NM_006661.4 | 99.9% | 100% | 864G>A;1779C>T |
| 2 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_011535388.3 | 99.9% | 100% | 864G>A;1779C>T |
| 3 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_024446311.1 | 99.9% | 100% | 864G>A;1779C>T |
| 4 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_006715321.4 | 98.8% | 98.5% | (many diffs) |
| 5 | human | 10846 | PDE10A | phosphodiesterase 10A | NM_001130690.2 | 97.6% | 97% | (many diffs) |
| 6 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_011535387.3 | 93.2% | 92.2% | (many diffs) |
| 7 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_017010195.2 | 93.1% | 93% | (many diffs) |
| 8 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_017010196.2 | 90.9% | 91% | 0_1ins210;654G>A;1569C>T |
| 9 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_024446312.1 | 90.9% | 91% | 0_1ins210;654G>A;1569C>T |
| 10 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_017010194.2 | 89.1% | 85.2% | (many diffs) |
| 11 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_017010197.2 | 67.5% | 64.9% | (many diffs) |
| 12 | human | 10846 | PDE10A | phosphodiesterase 10A | XM_011535393.3 | 59.3% | 58.7% | (many diffs) |
| 13 | human | 10846 | PDE10A | phosphodiesterase 10A | XR_001743121.2 | 22.2% | (many diffs) | |
| 14 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_017317461.1 | 85% | 92.9% | (many diffs) |
| 15 | mouse | 23984 | Pde10a | phosphodiesterase 10A | NM_001347321.1 | 85% | 93.1% | (many diffs) |
| 16 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523321.3 | 84.7% | 92.4% | (many diffs) |
| 17 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523319.3 | 84.5% | 91.1% | (many diffs) |
| 18 | mouse | 23984 | Pde10a | phosphodiesterase 10A | NM_011866.2 | 84% | 91.7% | (many diffs) |
| 19 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523320.3 | 83.9% | 91.9% | (many diffs) |
| 20 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523318.3 | 80.5% | 88% | (many diffs) |
| 21 | mouse | 23984 | Pde10a | phosphodiesterase 10A | NM_001290707.1 | 79% | 86.4% | (many diffs) |
| 22 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523316.3 | 75.3% | 82.3% | (many diffs) |
| 23 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_017317460.1 | 75.1% | 82.1% | (many diffs) |
| 24 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_006523323.1 | 71.3% | 79% | (many diffs) |
| 25 | mouse | 23984 | Pde10a | phosphodiesterase 10A | XM_017317462.1 | 25.8% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2406
- ORF length:
- 2337
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggatagaa gagaggaaat cccaacattt aacaggtttg acagatgaaa 121 aagtgaaggc atatctttct cttcaccccc aggtattaga tgaatttgta tctgaaagtg 181 ttagtgcaga gacagtagag aaatggctga agaggaagaa caacaaatca gaagatgaat 241 cagctcctaa ggaagtcagc aggtaccaag atacgaatat gcagggagtt gtatatgaac 301 taaacagcta tatagaacaa cggttggaca caggaggaga caaccagcta ctcctctatg 361 aactgagcag catcattaaa atagccacaa aagccgatgg atttgcactg tatttccttg 421 gagagtgcaa taatagcctg tgtatattca cgccacctgg gataaaggaa ggaaaacccc 481 gcctcatccc tgctgggccc atcactcagg gcaccaccgt ctctgcttat gtggccaagt 541 ccaggaaaac actgctagta gaagacatcc ttggagatga acgatttcca agaggtactg 601 gactggaatc agggactcgt atccagtctg ttctttgctt accaattgtc actgcaattg 661 gtgacttgat tggtattctc gagctgtatc ggcactgggg caaagaagcc ttctgtctta 721 gtcaccagga ggttgcaaca gcaaatcttg cctgggcttc agtagcaata catcaggtgc 781 aggtatgcag aggccttgcc aaacagacag aattgaatga cttcctactc gacgtatcaa 841 aaacatattt tgataacata gttgcaatag attctctact tgaacacata atgatatatg 901 caaaaaacct ggtgaatgcc gatcgttgtg cacttttcca ggtggaccat aagaacaagg 961 agttatattc agaccttttt gatattggag aggaaaagga aggaaaacct gtcttcaaga 1021 agaccaaaga gataagattt tcaattgaga aaggaattgc tggccaagta gcaagaacag 1081 gggaagtcct gaacattcca gatgcctatg cagacccacg ctttaacaga gaagtagact 1141 tgtacacagg ctacaccacg cggaacatcc tgtgcatgcc catcgtcagc cgaggcagcg 1201 tgataggtgt ggtgcagatg gtcaacaaaa tcagtggcag tgccttctct aaaacagatg 1261 aaaacaactt caaaatgttt gccgtctttt gtgctttagc cttacactgt gctaatatgt 1321 atcatagaat tcgccactca gagtgcattt accgggtaac gatggaaaag ctgtcctacc 1381 atagcatttg tacttcagaa gagtggcaag gtctcatgca attcaccctt cccgtgcgtc 1441 tctgcaaaga aattgaatta ttccactttg acattggtcc ttttgaaaac atgtggcctg 1501 gaatttttgt ctacatggtt catcggtcct gtgggacatc ctgctttgag cttgaaaagt 1561 tgtgtcgttt tattatgtct gtgaagaaga actatcggcg ggttccttat cacaactgga 1621 agcatgcggt cactgtagca cactgcatgt atgccatact tcagaacaat cacacgcttt 1681 tcacagacct tgagcgcaaa ggactgctga ttgcgtgtct gtgtcatgac ctggaccaca 1741 ggggcttcag taacagctac ctgcagaagt tcgaccaccc tctggccgct ctctactcca 1801 cttccaccat ggagcagcac cacttctccc agactgtgtc catccttcag ttggaagggc 1861 acaatatctt ctccactctg agctccagtg aatatgagca ggtgcttgag atcatccgca 1921 aagccatcat tgccacagac cttgctttat actttggaaa caggaagcag ttggaagaga 1981 tgtaccagac cggatcacta aaCCTTAATA ATCAATCACA TAGAGACCGT GTAATTGGTT 2041 TGATGATGAC TGCCTGTGAC CTTTGTTCTG TGACAAAACT GTGGCCCGTT ACAAAATTGA 2101 CGGCAAATGA TATATATGCA GAATTCTGGG CTGAGGGTGA TGAAATGAAG AAATTGGGAA 2161 TACAGCCTAT TCCTATGATG GACAGAGACA AGAAGGATGA AGTCCCCCAA GGCCAGCTTG 2221 GGTTCTACAA TGCCGTGGCC ATTCCCTGCT ATACAACCCT TACCCAGATC CTCCCTCCCA 2281 CGGAGCCTCT TCTGAAAGCA TGCAGGGATA ATCTCAGTCA GTGGGAGAAG GTGATTCGAG 2341 GGGAGGAGAC TGCAACCTGG ATTTCATCCC CATCCGTGGC TCAGAAGGCA GCTGCATCTG 2401 AAGATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2461 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2521 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGTGACATA CTACGCATGC AACGCACGCG 2581 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt