Construct: ORF TRCN0000477378

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000066.1_s317c1
Derived from:
ccsbBroadEn_07690
DNA Barcode:
CGTGACATACTACGCATGCAACGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE10A (10846)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477378
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10846 PDE10A phosphodiesterase 10A NM_006661.4 99.9% 100% 864G>A;1779C>T
2 human 10846 PDE10A phosphodiesterase 10A XM_011535388.3 99.9% 100% 864G>A;1779C>T
3 human 10846 PDE10A phosphodiesterase 10A XM_024446311.1 99.9% 100% 864G>A;1779C>T
4 human 10846 PDE10A phosphodiesterase 10A XM_006715321.4 98.8% 98.5% (many diffs)
5 human 10846 PDE10A phosphodiesterase 10A NM_001130690.2 97.6% 97% (many diffs)
6 human 10846 PDE10A phosphodiesterase 10A XM_011535387.3 93.2% 92.2% (many diffs)
7 human 10846 PDE10A phosphodiesterase 10A XM_017010195.2 93.1% 93% (many diffs)
8 human 10846 PDE10A phosphodiesterase 10A XM_017010196.2 90.9% 91% 0_1ins210;654G>A;1569C>T
9 human 10846 PDE10A phosphodiesterase 10A XM_024446312.1 90.9% 91% 0_1ins210;654G>A;1569C>T
10 human 10846 PDE10A phosphodiesterase 10A XM_017010194.2 89.1% 85.2% (many diffs)
11 human 10846 PDE10A phosphodiesterase 10A XM_017010197.2 67.5% 64.9% (many diffs)
12 human 10846 PDE10A phosphodiesterase 10A XM_011535393.3 59.3% 58.7% (many diffs)
13 human 10846 PDE10A phosphodiesterase 10A XR_001743121.2 22.2% (many diffs)
14 mouse 23984 Pde10a phosphodiesterase 10A XM_017317461.1 85% 92.9% (many diffs)
15 mouse 23984 Pde10a phosphodiesterase 10A NM_001347321.1 85% 93.1% (many diffs)
16 mouse 23984 Pde10a phosphodiesterase 10A XM_006523321.3 84.7% 92.4% (many diffs)
17 mouse 23984 Pde10a phosphodiesterase 10A XM_006523319.3 84.5% 91.1% (many diffs)
18 mouse 23984 Pde10a phosphodiesterase 10A NM_011866.2 84% 91.7% (many diffs)
19 mouse 23984 Pde10a phosphodiesterase 10A XM_006523320.3 83.9% 91.9% (many diffs)
20 mouse 23984 Pde10a phosphodiesterase 10A XM_006523318.3 80.5% 88% (many diffs)
21 mouse 23984 Pde10a phosphodiesterase 10A NM_001290707.1 79% 86.4% (many diffs)
22 mouse 23984 Pde10a phosphodiesterase 10A XM_006523316.3 75.3% 82.3% (many diffs)
23 mouse 23984 Pde10a phosphodiesterase 10A XM_017317460.1 75.1% 82.1% (many diffs)
24 mouse 23984 Pde10a phosphodiesterase 10A XM_006523323.1 71.3% 79% (many diffs)
25 mouse 23984 Pde10a phosphodiesterase 10A XM_017317462.1 25.8% 28% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2406
ORF length:
2337
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaggatagaa gagaggaaat cccaacattt aacaggtttg acagatgaaa
121aagtgaaggc atatctttct cttcaccccc aggtattaga tgaatttgta tctgaaagtg
181ttagtgcaga gacagtagag aaatggctga agaggaagaa caacaaatca gaagatgaat
241cagctcctaa ggaagtcagc aggtaccaag atacgaatat gcagggagtt gtatatgaac
301taaacagcta tatagaacaa cggttggaca caggaggaga caaccagcta ctcctctatg
361aactgagcag catcattaaa atagccacaa aagccgatgg atttgcactg tatttccttg
421gagagtgcaa taatagcctg tgtatattca cgccacctgg gataaaggaa ggaaaacccc
481gcctcatccc tgctgggccc atcactcagg gcaccaccgt ctctgcttat gtggccaagt
541ccaggaaaac actgctagta gaagacatcc ttggagatga acgatttcca agaggtactg
601gactggaatc agggactcgt atccagtctg ttctttgctt accaattgtc actgcaattg
661gtgacttgat tggtattctc gagctgtatc ggcactgggg caaagaagcc ttctgtctta
721gtcaccagga ggttgcaaca gcaaatcttg cctgggcttc agtagcaata catcaggtgc
781aggtatgcag aggccttgcc aaacagacag aattgaatga cttcctactc gacgtatcaa
841aaacatattt tgataacata gttgcaatag attctctact tgaacacata atgatatatg
901caaaaaacct ggtgaatgcc gatcgttgtg cacttttcca ggtggaccat aagaacaagg
961agttatattc agaccttttt gatattggag aggaaaagga aggaaaacct gtcttcaaga
1021agaccaaaga gataagattt tcaattgaga aaggaattgc tggccaagta gcaagaacag
1081gggaagtcct gaacattcca gatgcctatg cagacccacg ctttaacaga gaagtagact
1141tgtacacagg ctacaccacg cggaacatcc tgtgcatgcc catcgtcagc cgaggcagcg
1201tgataggtgt ggtgcagatg gtcaacaaaa tcagtggcag tgccttctct aaaacagatg
1261aaaacaactt caaaatgttt gccgtctttt gtgctttagc cttacactgt gctaatatgt
1321atcatagaat tcgccactca gagtgcattt accgggtaac gatggaaaag ctgtcctacc
1381atagcatttg tacttcagaa gagtggcaag gtctcatgca attcaccctt cccgtgcgtc
1441tctgcaaaga aattgaatta ttccactttg acattggtcc ttttgaaaac atgtggcctg
1501gaatttttgt ctacatggtt catcggtcct gtgggacatc ctgctttgag cttgaaaagt
1561tgtgtcgttt tattatgtct gtgaagaaga actatcggcg ggttccttat cacaactgga
1621agcatgcggt cactgtagca cactgcatgt atgccatact tcagaacaat cacacgcttt
1681tcacagacct tgagcgcaaa ggactgctga ttgcgtgtct gtgtcatgac ctggaccaca
1741ggggcttcag taacagctac ctgcagaagt tcgaccaccc tctggccgct ctctactcca
1801cttccaccat ggagcagcac cacttctccc agactgtgtc catccttcag ttggaagggc
1861acaatatctt ctccactctg agctccagtg aatatgagca ggtgcttgag atcatccgca
1921aagccatcat tgccacagac cttgctttat actttggaaa caggaagcag ttggaagaga
1981tgtaccagac cggatcacta aaCCTTAATA ATCAATCACA TAGAGACCGT GTAATTGGTT
2041TGATGATGAC TGCCTGTGAC CTTTGTTCTG TGACAAAACT GTGGCCCGTT ACAAAATTGA
2101CGGCAAATGA TATATATGCA GAATTCTGGG CTGAGGGTGA TGAAATGAAG AAATTGGGAA
2161TACAGCCTAT TCCTATGATG GACAGAGACA AGAAGGATGA AGTCCCCCAA GGCCAGCTTG
2221GGTTCTACAA TGCCGTGGCC ATTCCCTGCT ATACAACCCT TACCCAGATC CTCCCTCCCA
2281CGGAGCCTCT TCTGAAAGCA TGCAGGGATA ATCTCAGTCA GTGGGAGAAG GTGATTCGAG
2341GGGAGGAGAC TGCAACCTGG ATTTCATCCC CATCCGTGGC TCAGAAGGCA GCTGCATCTG
2401AAGATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2461TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2521CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGTGACATA CTACGCATGC AACGCACGCG
2581TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)