Transcript: Mouse XM_017318069.1

PREDICTED: Mus musculus kinesin light chain 2 (Klc2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Klc2 (16594)
Length:
3270
CDS:
501..2411

Additional Resources:

NCBI RefSeq record:
XM_017318069.1
NBCI Gene record:
Klc2 (16594)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318069.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100640 CCTCTCTCTTATCCAGTGGTA pLKO.1 2838 3UTR 100% 2.640 2.112 N Klc2 n/a
2 TRCN0000335046 CCTCTCTCTTATCCAGTGGTA pLKO_005 2838 3UTR 100% 2.640 2.112 N Klc2 n/a
3 TRCN0000100644 AGCTGGTACAAAGCCTGTAAA pLKO.1 1857 CDS 100% 13.200 9.240 N Klc2 n/a
4 TRCN0000334972 AGCTGGTACAAAGCCTGTAAA pLKO_005 1857 CDS 100% 13.200 9.240 N Klc2 n/a
5 TRCN0000100643 CAGCTCTCTTAACTTCCTTAA pLKO.1 2282 CDS 100% 10.800 7.560 N Klc2 n/a
6 TRCN0000100641 CCAGCTCTCTTAACTTCCTTA pLKO.1 2281 CDS 100% 4.950 3.465 N Klc2 n/a
7 TRCN0000335045 CCAGCTCTCTTAACTTCCTTA pLKO_005 2281 CDS 100% 4.950 3.465 N Klc2 n/a
8 TRCN0000100642 CCTGCTATTCATGAGTCAGAT pLKO.1 908 CDS 100% 4.950 3.465 N Klc2 n/a
9 TRCN0000335043 CCTGCTATTCATGAGTCAGAT pLKO_005 908 CDS 100% 4.950 3.465 N Klc2 n/a
10 TRCN0000348477 AGCTGAGTCAGGACGAGATAG pLKO_005 532 CDS 100% 10.800 6.480 N Klc2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318069.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03974 pDONR223 100% 85.4% 92.9% None (many diffs) n/a
2 ccsbBroad304_03974 pLX_304 0% 85.4% 92.9% V5 (many diffs) n/a
3 TRCN0000471275 GCCATTATGTATGACAGACTGTAC pLX_317 12.8% 85.4% 92.9% V5 (many diffs) n/a
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