Transcript: Mouse XM_017319473.1

PREDICTED: Mus musculus myocyte enhancer factor 2D (Mef2d), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mef2d (17261)
Length:
5480
CDS:
530..2050

Additional Resources:

NCBI RefSeq record:
XM_017319473.1
NBCI Gene record:
Mef2d (17261)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017319473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000310963 CAAAGGGTTAATGCATCATTT pLKO_005 1360 CDS 100% 13.200 18.480 N Mef2d n/a
2 TRCN0000085271 CAATGGCAACAGCCTAAACAA pLKO.1 1240 CDS 100% 5.625 7.875 N Mef2d n/a
3 TRCN0000085269 GCGAATCACTGATGAACGGAA pLKO.1 556 CDS 100% 2.640 2.112 N Mef2d n/a
4 TRCN0000310965 GCTGGATACTTGGACATTAAA pLKO_005 2026 CDS 100% 15.000 10.500 N Mef2d n/a
5 TRCN0000304479 GTCCTGTTGATGGTTACATTT pLKO_005 2166 3UTR 100% 13.200 9.240 N Mef2d n/a
6 TRCN0000085270 CACATCAGCATCAAGTCAGAA pLKO.1 1787 CDS 100% 4.950 3.465 N Mef2d n/a
7 TRCN0000085268 GCACTACAGAGAAACAGTGTT pLKO.1 1079 CDS 100% 4.950 3.465 N Mef2d n/a
8 TRCN0000315949 GCACTACAGAGAAACAGTGTT pLKO_005 1079 CDS 100% 4.950 3.465 N Mef2d n/a
9 TRCN0000085272 CCCTGGTGACATCATCCCTTA pLKO.1 1026 CDS 100% 4.050 2.835 N Mef2d n/a
10 TRCN0000349137 CCCTGGTGACATCATCCCTTA pLKO_005 1026 CDS 100% 4.050 2.835 N Mef2d n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017319473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00997 pDONR223 100% 86% 88.5% None (many diffs) n/a
2 ccsbBroad304_00997 pLX_304 0% 86% 88.5% V5 (many diffs) n/a
3 TRCN0000478896 TCTCGTATATTATACATCGTACAC pLX_317 23.4% 86% 88.5% V5 (many diffs) n/a
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