Construct: ORF TRCN0000478896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016345.1_s317c1
- Derived from:
- ccsbBroadEn_00997
- DNA Barcode:
- TCTCGTATATTATACATCGTACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEF2D (4209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4209 | MEF2D | myocyte enhancer factor 2D | NM_005920.4 | 99.8% | 100% | 282C>T;756A>G |
| 2 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_005245169.4 | 99.8% | 100% | 282C>T;756A>G |
| 3 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_005245170.3 | 99.8% | 100% | 282C>T;756A>G |
| 4 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_006711330.3 | 99.8% | 100% | 282C>T;756A>G |
| 5 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_011509569.3 | 99.8% | 100% | 282C>T;756A>G |
| 6 | human | 4209 | MEF2D | myocyte enhancer factor 2D | NM_001271629.2 | 98.5% | 98.6% | 282C>T;756A>G;853_854ins21 |
| 7 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_006711333.2 | 98.5% | 98.6% | 282C>T;756A>G;853_854ins21 |
| 8 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_017001314.1 | 98.5% | 98.6% | 282C>T;756A>G;853_854ins21 |
| 9 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_017001315.1 | 98.5% | 98.6% | 282C>T;756A>G;853_854ins21 |
| 10 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_006711332.3 | 94.6% | 94% | (many diffs) |
| 11 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XM_006711334.3 | 93.3% | 92.7% | (many diffs) |
| 12 | human | 4209 | MEF2D | myocyte enhancer factor 2D | XR_001737184.1 | 23.6% | (many diffs) | |
| 13 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | NM_001310587.1 | 90.9% | 95.9% | (many diffs) |
| 14 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_006501089.2 | 90.9% | 95.9% | (many diffs) |
| 15 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_017319472.1 | 90.9% | 95.9% | (many diffs) |
| 16 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | NM_133665.4 | 89.6% | 94.6% | (many diffs) |
| 17 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_011240032.2 | 89.6% | 94.6% | (many diffs) |
| 18 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_006501090.3 | 87.3% | 89.8% | (many diffs) |
| 19 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | NM_001310593.1 | 86% | 88.5% | (many diffs) |
| 20 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_017319473.1 | 86% | 88.5% | (many diffs) |
| 21 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_011240033.2 | 83% | 87.1% | (many diffs) |
| 22 | mouse | 17261 | Mef2d | myocyte enhancer factor 2D | XM_011240034.2 | 72.1% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaggaaaaag attcagatcc agcgaatcac cgacgagcgg aaccgacagg 121 tgactttcac caagcggaag tttggcctga tgaagaaggc gtatgagctg agcgtgctat 181 gtgactgcga gatcgcactc atcatcttca accactccaa caagctgttc cagtacgcca 241 gcaccgacat ggacaaggtg ctgctcaagt acacggagta caatgagcca cacgagagcc 301 gcaccaacgc cgacatcatc gagaccctga ggaagaaggg cttcaatggc tgcgacagcc 361 ccgagcccga cggggaggac tcgctggaac agagccccct gctggaggac aagtaccgac 421 gcgccagcga ggagctcgac gggctcttcc ggcgctatgg gtcaactgtc ccggccccca 481 actttgccat gcctgtcacg gtgcccgtgt ccaatcagag ctcactgcag ttcagcaatc 541 ccagcggctc cctggtcacc ccttccctgg tgacatcatc cctcacggac ccgcggctcc 601 tgtcccccca gcagccagca ctacagagga acagtgtgtc tcctggcctg ccccagcggc 661 cagctagtgc gggggccatg ctggggggtg acctgaacag tgctaacgga gcctgcccca 721 gccctgttgg gaatggctac gtcagtgctc gggcttcccc tggcctcctc cctgtggcca 781 atggcaacag cctaaacaag gtcatccctg ccaagtctcc gcccccacct acccacagca 841 cccagcttgg agcccccagc cgcaagcccg acctgcgagt catcacttcc caggcaggaa 901 aggggttaat gcatcacttg actgaggacc atttagatct gaacaatgcc cagcgccttg 961 gggtctccca gtctactcat tcgctcacca ccccagtggt ttctgtggca acgccgagtt 1021 tactcagcca gggcctcccc ttctcttcca tgcccactgc ctacaacaca gattaccagt 1081 tgaccagtgc agagctctcc tccttaccag cctttagttc acctgggggg ctgtcgctag 1141 gcaatgtcac tgcctggcaa cagccacagc agccccagca gccgcagcag ccacagcctc 1201 cacagcagca gccaccgcag ccacagcagc cacagccaca gcagcctcag cagccgcaac 1261 agccaccTCA GCAACAGTCC CACCTGGTCC CTGTATCTCT CAGCAACCTC ATCCCGGGCA 1321 GCCCCCTGCC CCACGTGGGT GCTGCCCTCA CAGTCACCAC CCACCCCCAC ATCAGCATCA 1381 AGTCAGAACC GGTGTCCCCA AGCCGTGAGC GCAGCCCTGC GCCTCCCCCT CCAGCTGTGT 1441 TCCCAGCTGC CCGCCCTGAG CCTGGCGATG GTCTCAGCAG CCCAGCCGGG GGATCCTATG 1501 AGACGGGAGA CCGGGATGAC GGACGGGGGG ACTTCGGGCC CACACTGGGC CTGCTGCGCC 1561 CAGCCCCAGA GCCTGAGGCT GAGGGCTCAG CTGTGAAGAG GATGCGGCTT GATACCTGGA 1621 CATTAAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1681 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1741 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCTCGT ATATTATACA TCGTACACAC 1801 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt