Transcript: Mouse XM_017322046.1

PREDICTED: Mus musculus protein tyrosine phosphatase, non-receptor type 5 (Ptpn5), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ptpn5 (19259)
Length:
3588
CDS:
933..2558

Additional Resources:

NCBI RefSeq record:
XM_017322046.1
NBCI Gene record:
Ptpn5 (19259)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017322046.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029905 GTGCGGAAGAATCGGTACAAA pLKO.1 1827 CDS 100% 5.625 7.875 N Ptpn5 n/a
2 TRCN0000029904 GCAGGCGGAATTCTTTGAAAT pLKO.1 1760 CDS 100% 0.000 0.000 N Ptpn5 n/a
3 TRCN0000029906 GATCCAGACATGCGAACAGTA pLKO.1 2474 CDS 100% 4.950 3.960 N Ptpn5 n/a
4 TRCN0000029907 CCTCTGAGTTCCTACATCAAT pLKO.1 1905 CDS 100% 5.625 3.938 N Ptpn5 n/a
5 TRCN0000029908 TCCTGCCTCATTGACTGTCAA pLKO.1 1544 CDS 100% 4.950 3.465 N Ptpn5 n/a
6 TRCN0000002664 GCTGCGACTCATCTCCCTCAA pLKO.1 2168 CDS 100% 1.350 0.945 N PTPN5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017322046.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09229 pDONR223 100% 84.8% 86.1% None (many diffs) n/a
2 ccsbBroad304_09229 pLX_304 0% 84.8% 86.1% V5 (many diffs) n/a
3 TRCN0000472997 GCTATGGCGAGTGCACGTAGATGT pLX_317 20.6% 84.8% 86.1% V5 (many diffs) n/a
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