Transcript: Human XM_024448217.1

PREDICTED: Homo sapiens calcium/calmodulin dependent protein kinase II gamma (CAMK2G), transcript variant X27, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CAMK2G (818)
Length:
2704
CDS:
256..1857

Additional Resources:

NCBI RefSeq record:
XM_024448217.1
NBCI Gene record:
CAMK2G (818)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448217.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199725 GCTCGGATATGTCGACTTCTG pLKO.1 248 5UTR 100% 4.050 5.670 N CAMK2G n/a
2 TRCN0000000480 GATCACCAGAAACTAGAACGT pLKO.1 224 5UTR 100% 2.640 3.696 N CAMK2G n/a
3 TRCN0000379741 GGGATGGATTTCCATAAGTTT pLKO_005 1510 CDS 100% 5.625 4.500 N CAMK2G n/a
4 TRCN0000000479 ACACAACGCTACAGATGGGAT pLKO.1 1185 CDS 100% 2.640 2.112 N CAMK2G n/a
5 TRCN0000382449 GCAGATGCCAGCCACTGTATA pLKO_005 340 CDS 100% 13.200 9.240 N CAMK2G n/a
6 TRCN0000218136 TCAGATTCTGGAGAGTGTTAA pLKO_005 363 CDS 100% 13.200 9.240 N CAMK2G n/a
7 TRCN0000382137 ACAGGAGATCATTAAGATTAC pLKO_005 1380 CDS 100% 10.800 7.560 N CAMK2G n/a
8 TRCN0000226387 AGAACGTGAGGCTCGGATATG pLKO_005 238 5UTR 100% 10.800 7.560 N CAMK2G n/a
9 TRCN0000381254 AGTGTTTGCGCAAGTTCAATG pLKO_005 875 CDS 100% 10.800 7.560 N CAMK2G n/a
10 TRCN0000380867 CGACTTCTGAAACATCCAAAC pLKO_005 260 CDS 100% 6.000 4.200 N CAMK2G n/a
11 TRCN0000024180 CCTGAGGTCTTGAGGAAAGAT pLKO.1 556 CDS 100% 5.625 3.938 N Camk2g n/a
12 TRCN0000381357 TGCTTGTCTCCAGGAACTTCT pLKO_005 932 CDS 100% 4.950 3.465 N CAMK2G n/a
13 TRCN0000381137 ACGCTACAGATGGGATCAAGG pLKO_005 1190 CDS 100% 4.050 2.835 N CAMK2G n/a
14 TRCN0000226388 TTGAGGCCTACACGAAGATTT pLKO_005 1436 CDS 100% 13.200 7.920 N CAMK2G n/a
15 TRCN0000361100 TTGAGGCCTACACGAAGATTT pLKO_005 1436 CDS 100% 13.200 7.920 N Camk2g n/a
16 TRCN0000382069 AGGATCAGCACAAGCTGTATC pLKO_005 659 CDS 100% 10.800 6.480 N CAMK2G n/a
17 TRCN0000024182 GAGGATCAGCACAAGCTGTAT pLKO.1 658 CDS 100% 4.950 2.970 N Camk2g n/a
18 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 1827 CDS 100% 4.950 2.475 Y GJD4 n/a
19 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 1827 CDS 100% 4.950 2.475 Y C9orf85 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448217.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14566 pDONR223 100% 65.1% 28% None (many diffs) n/a
2 ccsbBroad304_14566 pLX_304 0% 65.1% 28% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000480621 AAGACTGTGGCACCAACTCCCGCC pLX_317 29.4% 65.1% 28% V5 (not translated due to prior stop codon) (many diffs) n/a
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