Transcript: Human XM_024451585.1

PREDICTED: Homo sapiens aspartate dehydrogenase domain containing (ASPDH), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ASPDH (554235)
Length:
862
CDS:
31..732

Additional Resources:

NCBI RefSeq record:
XM_024451585.1
NBCI Gene record:
ASPDH (554235)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451585.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263273 CAAATCCTGCGCCATGCCAAT pLKO_005 286 CDS 100% 4.050 5.670 N ASPDH n/a
2 TRCN0000281585 TGATACCAGCCTCACGGACAT pLKO_005 525 CDS 100% 4.050 5.670 N ASPDH n/a
3 TRCN0000263274 CCTTGCACTGTGCTCTACGAA pLKO_005 391 CDS 100% 3.000 4.200 N ASPDH n/a
4 TRCN0000263275 CCCTGATCTGGTTGTGGAAGT pLKO_005 231 CDS 100% 4.050 2.835 N ASPDH n/a
5 TRCN0000263276 CCGCGAAATTCCAACACCATG pLKO_005 442 CDS 100% 4.050 2.835 N ASPDH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451585.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10184 pDONR223 100% 74.7% 70% None (many diffs) n/a
2 ccsbBroad304_10184 pLX_304 0% 74.7% 70% V5 (many diffs) n/a
3 TRCN0000472804 GTGATACCTCCTTCTCCCGACCCA pLX_317 76.5% 74.7% 70% V5 (many diffs) n/a
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