Construct: ORF TRCN0000472804
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000235.1_s317c1
- Derived from:
- ccsbBroadEn_10184
- DNA Barcode:
- GTGATACCTCCTTCTCCCGACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASPDH (554235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472804
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554235 | ASPDH | aspartate dehydrogenase dom... | NM_001024656.3 | 99.8% | 99.4% | 482A>G |
| 2 | human | 554235 | ASPDH | aspartate dehydrogenase dom... | XM_024451585.1 | 74.7% | 70% | (many diffs) |
| 3 | human | 554235 | ASPDH | aspartate dehydrogenase dom... | NM_001114598.2 | 61.5% | 57.9% | (many diffs) |
| 4 | mouse | 68352 | Aspdh | aspartate dehydrogenase dom... | NM_026690.1 | 52.6% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacagagtg aaaggtagca agtcaaggcg ccctgatctg gttgtggaag 121 tggcccatcc caaaataatc catgaatctg gggcacaaat cctgcgccat gccaatctcc 181 tgagccTTCG TGTCACCATG GCCACACACC CCGATGGCTT CCGGCTTGAG GGACCCCTGG 241 CTGCAGCCCA CAGCCCTGGG CCTTGCACTG TGCTCTACGA AGGCCCTGTC CGTGGGCTCT 301 GCCCCTTTGC CCCGCGAAAT TCCAACACCA TGGCGGCGGC TGCCCTGGCT GCCCCCAGCC 361 TGGGCTTCGA TGGGGTGATT GGGGTGCTCG TGGCTGATAC CAGCCTCACG GACATGCACG 421 TGGTGGATGT AGAGCTGAGC GGACCCCGGG GCCCCACGGG CCGAAGCTTT GCTGTGCACA 481 CCCGCAGAGA GAACCCTGCC GAGCCAGGCG CGGTCACCGG CTCCGCCACC GTCACGGCCT 541 TCTGGCGGAG CCTCCTGGCC TGCTGCCAGC TCCCCTCCAG GCCGGGGATC CATCTCTGCT 601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAGTGAT ACCTCCTTCT CCCGACCCAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt