Transcript: Human XM_024451632.1

PREDICTED: Homo sapiens zinc finger protein 350 (ZNF350), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF350 (59348)
Length:
2672
CDS:
542..2158

Additional Resources:

NCBI RefSeq record:
XM_024451632.1
NBCI Gene record:
ZNF350 (59348)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451632.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424359 TTGCTCGACTGTGTCAATTAA pLKO_005 2341 3UTR 100% 15.000 21.000 N ZNF350 n/a
2 TRCN0000419163 TTGGTGGGAAAGGGCTAATAC pLKO_005 2483 3UTR 100% 13.200 18.480 N ZNF350 n/a
3 TRCN0000108063 GCGTATATGTCGTGTCTGGTT pLKO.1 1793 CDS 100% 2.640 3.696 N ZNF350 n/a
4 TRCN0000108064 CAGAGCAAAGGCTATGAAATA pLKO.1 992 CDS 100% 13.200 9.240 N ZNF350 n/a
5 TRCN0000108060 CCTGATAAACTCATCCTATTA pLKO.1 2262 3UTR 100% 13.200 9.240 N ZNF350 n/a
6 TRCN0000429676 CTAACTGATCACCAGGTAATG pLKO_005 1220 CDS 100% 10.800 7.560 N ZNF350 n/a
7 TRCN0000108062 GCAAACAGGAACGTAGTCCTT pLKO.1 1997 CDS 100% 2.640 1.848 N ZNF350 n/a
8 TRCN0000108061 GCTAACCATGAACGACTTCAT pLKO.1 1058 CDS 100% 0.495 0.347 N ZNF350 n/a
9 TRCN0000012945 GCAGTGAATGTGGGAAAGCTT pLKO.1 1182 CDS 100% 3.000 1.500 Y ZNF146 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451632.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08786 pDONR223 100% 86.4% 86.4% None 0_1ins108;130_255del n/a
2 ccsbBroad304_08786 pLX_304 0% 86.4% 86.4% V5 0_1ins108;130_255del n/a
3 TRCN0000473440 TTTTCTGCAGACATACCTTTACCA pLX_317 32.6% 86.4% 86.4% V5 0_1ins108;130_255del n/a
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