Transcript: Human XM_024452211.1

PREDICTED: Homo sapiens transmembrane protein 184B (TMEM184B), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TMEM184B (25829)
Length:
3594
CDS:
592..1416

Additional Resources:

NCBI RefSeq record:
XM_024452211.1
NBCI Gene record:
TMEM184B (25829)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452211.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000150782 GAAAGACTTATTCCATCGGAT pLKO.1 656 CDS 100% 2.640 3.696 N TMEM184B n/a
2 TRCN0000156742 GTTCTTCATGGTCAAGTCCGT pLKO.1 915 CDS 100% 0.660 0.924 N TMEM184B n/a
3 TRCN0000421087 ATGGGCCTTCAGGAATATTTA pLKO_005 1594 3UTR 100% 15.000 10.500 N TMEM184B n/a
4 TRCN0000438414 GACACTGGGAGCAAGGCTTAT pLKO_005 1653 3UTR 100% 10.800 7.560 N TMEM184B n/a
5 TRCN0000158117 CATGTCGGAGATCAGAGGAAA pLKO.1 591 5UTR 100% 4.950 3.465 N TMEM184B n/a
6 TRCN0000153494 CGTGACCATCATCTACAACAT pLKO.1 810 CDS 100% 4.950 3.465 N TMEM184B n/a
7 TRCN0000157884 CAACGACCAGTACTACGTGTA pLKO.1 415 5UTR 100% 4.050 2.835 N TMEM184B n/a
8 TRCN0000157290 CCATCGGATTTCTGAGGTTCT pLKO.1 668 CDS 100% 4.050 2.835 N TMEM184B n/a
9 TRCN0000153824 CCTCAAGTTCTTCATGGTCAA pLKO.1 909 CDS 100% 4.050 2.835 N TMEM184B n/a
10 TRCN0000154251 CATCTTTCTTTCCTTCTGGCA pLKO.1 936 CDS 100% 0.660 0.396 N TMEM184B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452211.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02867 pDONR223 100% 64.4% 64.4% None 0_1ins420;562_582del n/a
2 ccsbBroad304_02867 pLX_304 0% 64.4% 64.4% V5 0_1ins420;562_582del n/a
3 TRCN0000473765 TGCTATGCCCGCCGTCACCCCTTT pLX_317 33.1% 64.4% 64.4% V5 0_1ins420;562_582del n/a
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