Construct: ORF TRCN0000473765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017955.1_s317c1
- Derived from:
- ccsbBroadEn_02867
- DNA Barcode:
- TGCTATGCCCGCCGTCACCCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM184B (25829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473765
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25829 | TMEM184B | transmembrane protein 184B | NM_001195071.1 | 100% | 100% | |
| 2 | human | 25829 | TMEM184B | transmembrane protein 184B | NM_012264.5 | 100% | 100% | |
| 3 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_017028756.1 | 98.3% | 98.3% | 982_1002del |
| 4 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530113.1 | 97.1% | 97.1% | 981_1016del |
| 5 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530114.1 | 97.1% | 97.1% | 981_1016del |
| 6 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_017028755.1 | 92.5% | 92.5% | 1_99del |
| 7 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530112.2 | 91% | 91% | 1_99del;1081_1101del |
| 8 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_017028754.2 | 90% | 90% | 1_99del;1080_1115del |
| 9 | human | 25829 | TMEM184B | transmembrane protein 184B | NM_001195072.2 | 83.7% | 83.7% | 0_1ins198 |
| 10 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530117.1 | 81.3% | 81.3% | 0_1ins198;783_818del |
| 11 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530118.1 | 81.3% | 81.3% | 0_1ins198;783_818del |
| 12 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_017028757.1 | 78.9% | 67.6% | 1_94del;285_286ins166;910_930del |
| 13 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530115.3 | 78% | 66.8% | 1_94del;285_286ins166;909_944del |
| 14 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_017028759.1 | 71.1% | 70.7% | (many diffs) |
| 15 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_011530119.1 | 69.1% | 68.7% | (many diffs) |
| 16 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_024452211.1 | 64.4% | 64.4% | 0_1ins420;562_582del |
| 17 | human | 25829 | TMEM184B | transmembrane protein 184B | XM_024452210.1 | 63.7% | 63.7% | 0_1ins420;561_596del |
| 18 | mouse | 223693 | Tmem184b | transmembrane protein 184b | NM_001253817.1 | 91% | 96% | (many diffs) |
| 19 | mouse | 223693 | Tmem184b | transmembrane protein 184b | NM_172608.1 | 91% | 96% | (many diffs) |
| 20 | mouse | 223693 | Tmem184b | transmembrane protein 184b | NM_001253819.1 | 89.5% | 94.4% | (many diffs) |
| 21 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_006520806.2 | 89.5% | 94.4% | (many diffs) |
| 22 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_006520805.3 | 85% | 89.6% | (many diffs) |
| 23 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_006520804.2 | 83.6% | 88.2% | (many diffs) |
| 24 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_006520807.3 | 75.6% | 80.1% | (many diffs) |
| 25 | mouse | 223693 | Tmem184b | transmembrane protein 184b | NM_001253820.1 | 59.9% | 64.3% | (many diffs) |
| 26 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_011245585.2 | 58.9% | 63.2% | (many diffs) |
| 27 | mouse | 223693 | Tmem184b | transmembrane protein 184b | XM_006520810.3 | 55.4% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1287
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agtgaggggg gatgtgctgg ccccggatcc agcgtcgccc acgaccgcag 121 cagcctcgcc cagcgtctcc gtgatccccg agggcagccc cactgccatg gagcagcctg 181 tgttcctgat gacaactgcc gctcaggcca tctctggctt cttcgtgtgg acggccctgc 241 tcatcacatg ccaccagatc tacatgcacc tgcgctgcta cagctgcccc aacgagcagc 301 gctacatcgt gcgcatcctc ttcatcgtgc ccatctacgc ctttgactcc tggctcagcc 361 tcctcttctt caccaacgac cagtactacg tgtacttcgg caccgtccgc gactgctatg 421 aggccttggt catctataat ttcctgagcc tgtgctatga gtacctagga ggagaaagtt 481 ccatcatgtc ggagatcaga ggaaaaccca ttgagtccag ctgtatgtat ggcacctgct 541 gcctctgggg aaagacttat tccatcggat ttctgaggtt ctgcaaacag gccaccctgc 601 agttctgtgt ggtgaagcca ctcatggcgg tcagcactgt ggtcctccag gccttcggca 661 agtaccggga tggggacttt gacgtcacca gtggctacct ctacgtgacc atcatctaca 721 acatctccgt cagcctggcc ctctacgccc tcttcctctt ctacttcgcc acccgggagc 781 tgctcagccc ctacagcccc gtcctcaagt tcttcatggt caagtccgtc atctttcttt 841 ccttctggca aggcatgctc ctggccatcc tggagaagtg tggggCCATC CCCAAAATCC 901 ACTCGGCCCG CGTGTCGGTG GGCGAGGGCA CCGTGGCTGC CGGCTACCAG GACTTCATCA 961 TCTGTGTGGA GATGTTCTTT GCAGCCCTGG CCCTGCGGCA CGCCTTCACC TACAAGGTCT 1021 ATGCTGACAA GAGGCTGGAC GCACAAGGCC GCTGTGCCCC CATGAAGAGC ATCTCCAGCA 1081 GCCTCAAGGA GACCATGAAC CCGCACGACA TCGTGCAGGA CGCCATCCAC AACTTCTCAC 1141 CTGCCTACCA GCAGTACACG CAGCAGTCCA CCCTGGAGCC TGGGCCCACC TGGCGTGGTG 1201 GCGCCCACGG CCTCTCCCGC TCCCACAGCC TCAGTGGCGC CCGCGACAAC GAGAAGACTC 1261 TCCTGCTCAG CTCTGATGAT GAATTCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGCTATGC 1441 CCGCCGTCAC CCCTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt