Construct: ORF TRCN0000473765

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017955.1_s317c1
Derived from:
ccsbBroadEn_02867
DNA Barcode:
TGCTATGCCCGCCGTCACCCCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM184B (25829)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473765
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25829 TMEM184B transmembrane protein 184B NM_001195071.1 100% 100%
2 human 25829 TMEM184B transmembrane protein 184B NM_012264.5 100% 100%
3 human 25829 TMEM184B transmembrane protein 184B XM_017028756.1 98.3% 98.3% 982_1002del
4 human 25829 TMEM184B transmembrane protein 184B XM_011530113.1 97.1% 97.1% 981_1016del
5 human 25829 TMEM184B transmembrane protein 184B XM_011530114.1 97.1% 97.1% 981_1016del
6 human 25829 TMEM184B transmembrane protein 184B XM_017028755.1 92.5% 92.5% 1_99del
7 human 25829 TMEM184B transmembrane protein 184B XM_011530112.2 91% 91% 1_99del;1081_1101del
8 human 25829 TMEM184B transmembrane protein 184B XM_017028754.2 90% 90% 1_99del;1080_1115del
9 human 25829 TMEM184B transmembrane protein 184B NM_001195072.2 83.7% 83.7% 0_1ins198
10 human 25829 TMEM184B transmembrane protein 184B XM_011530117.1 81.3% 81.3% 0_1ins198;783_818del
11 human 25829 TMEM184B transmembrane protein 184B XM_011530118.1 81.3% 81.3% 0_1ins198;783_818del
12 human 25829 TMEM184B transmembrane protein 184B XM_017028757.1 78.9% 67.6% 1_94del;285_286ins166;910_930del
13 human 25829 TMEM184B transmembrane protein 184B XM_011530115.3 78% 66.8% 1_94del;285_286ins166;909_944del
14 human 25829 TMEM184B transmembrane protein 184B XM_017028759.1 71.1% 70.7% (many diffs)
15 human 25829 TMEM184B transmembrane protein 184B XM_011530119.1 69.1% 68.7% (many diffs)
16 human 25829 TMEM184B transmembrane protein 184B XM_024452211.1 64.4% 64.4% 0_1ins420;562_582del
17 human 25829 TMEM184B transmembrane protein 184B XM_024452210.1 63.7% 63.7% 0_1ins420;561_596del
18 mouse 223693 Tmem184b transmembrane protein 184b NM_001253817.1 91% 96% (many diffs)
19 mouse 223693 Tmem184b transmembrane protein 184b NM_172608.1 91% 96% (many diffs)
20 mouse 223693 Tmem184b transmembrane protein 184b NM_001253819.1 89.5% 94.4% (many diffs)
21 mouse 223693 Tmem184b transmembrane protein 184b XM_006520806.2 89.5% 94.4% (many diffs)
22 mouse 223693 Tmem184b transmembrane protein 184b XM_006520805.3 85% 89.6% (many diffs)
23 mouse 223693 Tmem184b transmembrane protein 184b XM_006520804.2 83.6% 88.2% (many diffs)
24 mouse 223693 Tmem184b transmembrane protein 184b XM_006520807.3 75.6% 80.1% (many diffs)
25 mouse 223693 Tmem184b transmembrane protein 184b NM_001253820.1 59.9% 64.3% (many diffs)
26 mouse 223693 Tmem184b transmembrane protein 184b XM_011245585.2 58.9% 63.2% (many diffs)
27 mouse 223693 Tmem184b transmembrane protein 184b XM_006520810.3 55.4% 54.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1287
ORF length:
1221
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac agtgaggggg gatgtgctgg ccccggatcc agcgtcgccc acgaccgcag
121cagcctcgcc cagcgtctcc gtgatccccg agggcagccc cactgccatg gagcagcctg
181tgttcctgat gacaactgcc gctcaggcca tctctggctt cttcgtgtgg acggccctgc
241tcatcacatg ccaccagatc tacatgcacc tgcgctgcta cagctgcccc aacgagcagc
301gctacatcgt gcgcatcctc ttcatcgtgc ccatctacgc ctttgactcc tggctcagcc
361tcctcttctt caccaacgac cagtactacg tgtacttcgg caccgtccgc gactgctatg
421aggccttggt catctataat ttcctgagcc tgtgctatga gtacctagga ggagaaagtt
481ccatcatgtc ggagatcaga ggaaaaccca ttgagtccag ctgtatgtat ggcacctgct
541gcctctgggg aaagacttat tccatcggat ttctgaggtt ctgcaaacag gccaccctgc
601agttctgtgt ggtgaagcca ctcatggcgg tcagcactgt ggtcctccag gccttcggca
661agtaccggga tggggacttt gacgtcacca gtggctacct ctacgtgacc atcatctaca
721acatctccgt cagcctggcc ctctacgccc tcttcctctt ctacttcgcc acccgggagc
781tgctcagccc ctacagcccc gtcctcaagt tcttcatggt caagtccgtc atctttcttt
841ccttctggca aggcatgctc ctggccatcc tggagaagtg tggggCCATC CCCAAAATCC
901ACTCGGCCCG CGTGTCGGTG GGCGAGGGCA CCGTGGCTGC CGGCTACCAG GACTTCATCA
961TCTGTGTGGA GATGTTCTTT GCAGCCCTGG CCCTGCGGCA CGCCTTCACC TACAAGGTCT
1021ATGCTGACAA GAGGCTGGAC GCACAAGGCC GCTGTGCCCC CATGAAGAGC ATCTCCAGCA
1081GCCTCAAGGA GACCATGAAC CCGCACGACA TCGTGCAGGA CGCCATCCAC AACTTCTCAC
1141CTGCCTACCA GCAGTACACG CAGCAGTCCA CCCTGGAGCC TGGGCCCACC TGGCGTGGTG
1201GCGCCCACGG CCTCTCCCGC TCCCACAGCC TCAGTGGCGC CCGCGACAAC GAGAAGACTC
1261TCCTGCTCAG CTCTGATGAT GAATTCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG
1321GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1381AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGCTATGC
1441CCGCCGTCAC CCCTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1501aagatt

Download FASTA (ORF) (Full)