Transcript: Human XM_024452430.1

PREDICTED: Homo sapiens TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Length:
4886
CDS:
11..4828

Additional Resources:

NCBI RefSeq record:
XM_024452430.1
NBCI Gene record:
TAF1 (6872)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145911 TCTGGTATATGGACGCTGGG pXPR_003 AGG 1441 30% 9 0.0488 TAF1 TAF1 77870
2 BRDN0001147230 TATTATTATCCCAAGCAACA pXPR_003 GGG 1733 36% 11 0.0057 TAF1 TAF1 77869
3 BRDN0001145163 GACCAGGATTCTATTACTGG pXPR_003 TGG 467 10% 4 -0.4393 TAF1 TAF1 77871
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006285 CCTTCTAGCATGACTAGGAAT pLKO.1 1385 CDS 100% 4.950 6.930 N TAF1 n/a
2 TRCN0000006288 GCCACTCTTGATGATGACAAA pLKO.1 1439 CDS 100% 4.950 3.465 N TAF1 n/a
3 TRCN0000280260 GCCACTCTTGATGATGACAAA pLKO_005 1439 CDS 100% 4.950 3.465 N TAF1 n/a
4 TRCN0000006286 GCTGCAAGCATTTGAGAACAA pLKO.1 2290 CDS 100% 4.950 3.465 N TAF1 n/a
5 TRCN0000195160 CCACTGTTCACTGTGACTATT pLKO.1 4152 CDS 100% 13.200 7.920 N TAF1 n/a
6 TRCN0000280315 CCACTGTTCACTGTGACTATT pLKO_005 4152 CDS 100% 13.200 7.920 N TAF1 n/a
7 TRCN0000006287 CCAATGGATTTAGAGACCATA pLKO.1 4712 CDS 100% 4.950 2.970 N TAF1 n/a
8 TRCN0000196898 GCAAAGATGGTGATCTTATTC pLKO.1 2091 CDS 100% 1.320 0.792 N TAF1 n/a
9 TRCN0000196851 GCCAACAGTGTTAAGTATAAT pLKO.1 4802 CDS 100% 15.000 7.500 Y TAF1L n/a
10 TRCN0000349745 GCCAACAGTGTTAAGTATAAT pLKO_005 4802 CDS 100% 15.000 7.500 Y TAF1L n/a
11 TRCN0000195039 CCAAGCAACTTCTACGTAAAT pLKO.1 3081 CDS 100% 13.200 6.600 Y TAF1L n/a
12 TRCN0000196373 GTTACTATATTCGGGAATTAG pLKO.1 2382 CDS 100% 13.200 6.600 Y TAF1L n/a
13 TRCN0000312663 GTTACTATATTCGGGAATTAG pLKO_005 2382 CDS 100% 13.200 6.600 Y TAF1L n/a
14 TRCN0000079049 GCTGGGATTATGCAGCATGAT pLKO.1 707 CDS 100% 4.950 2.475 Y Taf1 n/a
15 TRCN0000037502 CCAGCATTCAATTCCTGCTAT pLKO.1 1840 CDS 100% 4.950 2.475 Y TAF1L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.