Transcript: Human XM_024453217.1

PREDICTED: Homo sapiens CD8b2 molecule (CD8B2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CD8B2 (927)
Length:
4926
CDS:
93..833

Additional Resources:

NCBI RefSeq record:
XM_024453217.1
NBCI Gene record:
CD8B2 (927)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453217.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000371762 TCAGCTGAGTGTGGTTGATTT pLKO_005 482 CDS 100% 13.200 6.600 Y CD8B n/a
2 TRCN0000371712 CCTGGTAGGGCAGTAACATTG pLKO_005 1140 3UTR 100% 10.800 5.400 Y CD8B n/a
3 TRCN0000057508 GCTGGAGGACTGAGTAAGAAA pLKO.1 1024 3UTR 100% 5.625 2.813 Y CD8B n/a
4 TRCN0000057512 GCAAGCCGGTTCATTCTCAAT pLKO.1 378 CDS 100% 4.950 2.475 Y CD8B n/a
5 TRCN0000180418 GATCACTTGAGCTCAGGAGTT pLKO.1 4348 3UTR 100% 4.050 2.025 Y ERN2 n/a
6 TRCN0000057509 CCTGCATACATAAAGGTGCAA pLKO.1 168 CDS 100% 2.640 1.320 Y CD8B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453217.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05956 pDONR223 100% 87.1% 83.7% None (many diffs) n/a
2 ccsbBroad304_05956 pLX_304 0% 87.1% 83.7% V5 (many diffs) n/a
3 TRCN0000466988 TTTGATACTTCGTTTTAAGAATTT pLX_317 48.5% 87.1% 83.7% V5 (many diffs) n/a
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