Transcript: Human XM_024453290.1

PREDICTED: Homo sapiens RNA binding motif protein 5 (RBM5), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RBM5 (10181)
Length:
5804
CDS:
3767..5296

Additional Resources:

NCBI RefSeq record:
XM_024453290.1
NBCI Gene record:
RBM5 (10181)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453290.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000349734 ACTATGGTGAGCACGACTATA pLKO_005 371 5UTR 100% 13.200 18.480 N RBM5 n/a
2 TRCN0000312602 ACCATCACAGAGAGCGATATT pLKO_005 451 5UTR 100% 13.200 9.240 N RBM5 n/a
3 TRCN0000074874 CGCGTCTTTAGCTGTCAATAA pLKO.1 3787 CDS 100% 13.200 9.240 N RBM5 n/a
4 TRCN0000369392 TGACCGTGATGAGCGTGAATC pLKO_005 198 5UTR 100% 10.800 7.560 N RBM5 n/a
5 TRCN0000054665 CCACAGTAACATTGGCAACAA pLKO.1 5074 CDS 100% 4.950 3.465 N Rbm5 n/a
6 TRCN0000287688 CCACAGTAACATTGGCAACAA pLKO_005 5074 CDS 100% 4.950 3.465 N Rbm5 n/a
7 TRCN0000074875 CCCAGACCTAAGTTTGAAGAT pLKO.1 3618 5UTR 100% 4.950 3.465 N RBM5 n/a
8 TRCN0000327736 CCCAGACCTAAGTTTGAAGAT pLKO_005 3618 5UTR 100% 4.950 3.465 N RBM5 n/a
9 TRCN0000074873 CCCTCCCACCTTAAAGAAGTT pLKO.1 5437 3UTR 100% 4.950 3.465 N RBM5 n/a
10 TRCN0000074877 GCTGGAGGATTGGAATCTGAT pLKO.1 4181 CDS 100% 4.950 3.465 N RBM5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453290.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11465 pDONR223 100% 29.4% 29.4% None (many diffs) n/a
2 ccsbBroad304_11465 pLX_304 0% 29.4% 29.4% V5 (many diffs) n/a
3 TRCN0000466364 TTAGGTTTCCAGCGTTGTGTGGTT pLX_317 18.9% 29.4% 29.4% V5 (many diffs) n/a
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