Construct: ORF TRCN0000466364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012550.1_s317c1
- Derived from:
- ccsbBroadEn_11465
- DNA Barcode:
- TTAGGTTTCCAGCGTTGTGTGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM5 (10181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_011533262.2 | 73.3% | 73.1% | (many diffs) |
| 2 | human | 10181 | RBM5 | RNA binding motif protein 5 | NM_005778.4 | 66.9% | 66.9% | 990T>C;1639_2445del |
| 3 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_017005503.1 | 66.9% | 66.9% | 990T>C;1639_2445del |
| 4 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_006712917.2 | 59.7% | 59.7% | 990T>C;1278_1279ins177;1462_2268del |
| 5 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_011533261.2 | 57.8% | 55.9% | (many diffs) |
| 6 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_017005504.2 | 50.6% | 48.7% | (many diffs) |
| 7 | human | 10181 | RBM5 | RNA binding motif protein 5 | XR_427245.2 | 49.7% | (many diffs) | |
| 8 | human | 10181 | RBM5 | RNA binding motif protein 5 | NR_036627.3 | 49.1% | (many diffs) | |
| 9 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_017005505.1 | 36.6% | 36.6% | 0_1ins741;249T>C;898_1704del |
| 10 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_024453289.1 | 36.6% | 36.6% | 0_1ins741;249T>C;898_1704del |
| 11 | human | 10181 | RBM5 | RNA binding motif protein 5 | XM_024453290.1 | 29.4% | 29.4% | (many diffs) |
| 12 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | NM_148930.3 | 61.7% | 65.2% | (many diffs) |
| 13 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511870.3 | 61.7% | 65.2% | (many diffs) |
| 14 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511871.3 | 61.7% | 65.2% | (many diffs) |
| 15 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511872.2 | 61.7% | 65.2% | (many diffs) |
| 16 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_011242921.2 | 61.6% | 65.1% | (many diffs) |
| 17 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XR_001779079.1 | 46.1% | (many diffs) | |
| 18 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511874.2 | 41.7% | 44.1% | (many diffs) |
| 19 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XR_001779080.1 | 40.4% | (many diffs) | |
| 20 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511875.3 | 32.8% | 35% | (many diffs) |
| 21 | mouse | 83486 | Rbm5 | RNA binding motif protein 5 | XM_006511876.1 | 32.8% | 35% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1704
- ORF length:
- 1638
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ttcagacaaa agagtgagta gaacagagcg tagtggaaga tacggttcca 121 tcatagacag ggatgaccgt gatgagcgtg aatcccgaag caggcggagg gactcagatt 181 acaaaagatc tagtgatgat cggaggggtg atagatatga tgactaccga gactatgaca 241 gtccagagag agagcgtgaa agaaggaaca gtgaccgatc cgaagatggc taccattcag 301 atggtgacta tggtgagcac gactataggc atgacatcag tgacgagagg gagagcaaga 361 ccatcatgct gcgcggcctt cccatcacca tcacagagag cgatattcga gaaatgatgg 421 agtccttcga aggccctcag cctgcggatg tgaggctgat gaagaggaaa acaggtgtaa 481 gccgtggttt cgccttcgtg gagttttatc acttgcaaga tgctaccagc tggatggaag 541 ccaatcagaa aaagttggtg attcaaggaa agcacattgc aatgcattat agcaatccca 601 gacctaagtt tgaagattgg ctttgtaaca agtgctgcct taacaatttc aggaaaagac 661 taaaatgctt ccgatgtgga gcagacaagt ttgactctga acaggaagtg cctcctggaa 721 ccacagagtc ggttcagtct gtggattact actgtgatac gatcattctt cggaacatag 781 ctccgcacac tgtggtggat tccatcatga cagcactgtc tccttacgcg tctttagctg 841 tcaataacat ccgcctcata aaagacaaac agacccagca gaacagaggc ttcgcatttg 901 tgcagctgtc ctctgcaatg gatgcttctc agctgcttca gatattacag agtctccatc 961 ctcctttgaa aattgatggc aaaactattg gggttgattt tgcaaaaagt gccagaaaag 1021 acttggtcct ctcagatggt aaccgcgtca gcgccttctc tgtagctagt acggctattg 1081 ctgctgctca gtggtcatcc acccagtctc aaagtggtga aggaggcagt gttgactaca 1141 gttatctgca accaggtcaa gatggctatg cccaatatgc tcagtattca caggattatc 1201 agcagtttta tcaacaacaa gctggaggat tggaatctga tgcatcatct gcatcaggca 1261 cagcagtgac caccacctca gcggctgtag tgtcccagag tcctcagctg tataatcaaa 1321 cctccaatcc acctggctct ccgactgagg aagcacagcc tagcactagc acaagtacac 1381 aggccccagc cgcttccccT ACTGGTGTAG TTCCTGGTAC CAAATATGCA GTACCTGACA 1441 CGTCCACTTA CCAGTATGAT GAATCTTCAG GATATTACTA TGATCCGACA ACAGGGCTCT 1501 ATTATGACCC CAACTCGCAA TACTACTATA ATTCCTTGAC CCAGCAGTAC CTTTACTGGG 1561 ATGGGGAAAA AGAGACCTAC GTGCCAGCTG CAGAGTCTAG CTCCCACCAG CAGTCGGGCC 1621 TGCCTCCTGC AAAAGAGGGG AAAGAGAAGA AGGAGAAACC CAAGAGCAAA ACAGCCCAGC 1681 AGATTGCCAA AGACATGGAA CGCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1741 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCGTAACT 1801 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT AGGTTTCCAG 1861 CGTTGTGTGG TTACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1921 tt