Construct: ORF TRCN0000466364

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012550.1_s317c1
Derived from:
ccsbBroadEn_11465
DNA Barcode:
TTAGGTTTCCAGCGTTGTGTGGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RBM5 (10181)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466364
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10181 RBM5 RNA binding motif protein 5 XM_011533262.2 73.3% 73.1% (many diffs)
2 human 10181 RBM5 RNA binding motif protein 5 NM_005778.4 66.9% 66.9% 990T>C;1639_2445del
3 human 10181 RBM5 RNA binding motif protein 5 XM_017005503.1 66.9% 66.9% 990T>C;1639_2445del
4 human 10181 RBM5 RNA binding motif protein 5 XM_006712917.2 59.7% 59.7% 990T>C;1278_1279ins177;1462_2268del
5 human 10181 RBM5 RNA binding motif protein 5 XM_011533261.2 57.8% 55.9% (many diffs)
6 human 10181 RBM5 RNA binding motif protein 5 XM_017005504.2 50.6% 48.7% (many diffs)
7 human 10181 RBM5 RNA binding motif protein 5 XR_427245.2 49.7% (many diffs)
8 human 10181 RBM5 RNA binding motif protein 5 NR_036627.3 49.1% (many diffs)
9 human 10181 RBM5 RNA binding motif protein 5 XM_017005505.1 36.6% 36.6% 0_1ins741;249T>C;898_1704del
10 human 10181 RBM5 RNA binding motif protein 5 XM_024453289.1 36.6% 36.6% 0_1ins741;249T>C;898_1704del
11 human 10181 RBM5 RNA binding motif protein 5 XM_024453290.1 29.4% 29.4% (many diffs)
12 mouse 83486 Rbm5 RNA binding motif protein 5 NM_148930.3 61.7% 65.2% (many diffs)
13 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511870.3 61.7% 65.2% (many diffs)
14 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511871.3 61.7% 65.2% (many diffs)
15 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511872.2 61.7% 65.2% (many diffs)
16 mouse 83486 Rbm5 RNA binding motif protein 5 XM_011242921.2 61.6% 65.1% (many diffs)
17 mouse 83486 Rbm5 RNA binding motif protein 5 XR_001779079.1 46.1% (many diffs)
18 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511874.2 41.7% 44.1% (many diffs)
19 mouse 83486 Rbm5 RNA binding motif protein 5 XR_001779080.1 40.4% (many diffs)
20 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511875.3 32.8% 35% (many diffs)
21 mouse 83486 Rbm5 RNA binding motif protein 5 XM_006511876.1 32.8% 35% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1704
ORF length:
1638
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ttcagacaaa agagtgagta gaacagagcg tagtggaaga tacggttcca
121tcatagacag ggatgaccgt gatgagcgtg aatcccgaag caggcggagg gactcagatt
181acaaaagatc tagtgatgat cggaggggtg atagatatga tgactaccga gactatgaca
241gtccagagag agagcgtgaa agaaggaaca gtgaccgatc cgaagatggc taccattcag
301atggtgacta tggtgagcac gactataggc atgacatcag tgacgagagg gagagcaaga
361ccatcatgct gcgcggcctt cccatcacca tcacagagag cgatattcga gaaatgatgg
421agtccttcga aggccctcag cctgcggatg tgaggctgat gaagaggaaa acaggtgtaa
481gccgtggttt cgccttcgtg gagttttatc acttgcaaga tgctaccagc tggatggaag
541ccaatcagaa aaagttggtg attcaaggaa agcacattgc aatgcattat agcaatccca
601gacctaagtt tgaagattgg ctttgtaaca agtgctgcct taacaatttc aggaaaagac
661taaaatgctt ccgatgtgga gcagacaagt ttgactctga acaggaagtg cctcctggaa
721ccacagagtc ggttcagtct gtggattact actgtgatac gatcattctt cggaacatag
781ctccgcacac tgtggtggat tccatcatga cagcactgtc tccttacgcg tctttagctg
841tcaataacat ccgcctcata aaagacaaac agacccagca gaacagaggc ttcgcatttg
901tgcagctgtc ctctgcaatg gatgcttctc agctgcttca gatattacag agtctccatc
961ctcctttgaa aattgatggc aaaactattg gggttgattt tgcaaaaagt gccagaaaag
1021acttggtcct ctcagatggt aaccgcgtca gcgccttctc tgtagctagt acggctattg
1081ctgctgctca gtggtcatcc acccagtctc aaagtggtga aggaggcagt gttgactaca
1141gttatctgca accaggtcaa gatggctatg cccaatatgc tcagtattca caggattatc
1201agcagtttta tcaacaacaa gctggaggat tggaatctga tgcatcatct gcatcaggca
1261cagcagtgac caccacctca gcggctgtag tgtcccagag tcctcagctg tataatcaaa
1321cctccaatcc acctggctct ccgactgagg aagcacagcc tagcactagc acaagtacac
1381aggccccagc cgcttccccT ACTGGTGTAG TTCCTGGTAC CAAATATGCA GTACCTGACA
1441CGTCCACTTA CCAGTATGAT GAATCTTCAG GATATTACTA TGATCCGACA ACAGGGCTCT
1501ATTATGACCC CAACTCGCAA TACTACTATA ATTCCTTGAC CCAGCAGTAC CTTTACTGGG
1561ATGGGGAAAA AGAGACCTAC GTGCCAGCTG CAGAGTCTAG CTCCCACCAG CAGTCGGGCC
1621TGCCTCCTGC AAAAGAGGGG AAAGAGAAGA AGGAGAAACC CAAGAGCAAA ACAGCCCAGC
1681AGATTGCCAA AGACATGGAA CGCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1741AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCGTAACT
1801TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT AGGTTTCCAG
1861CGTTGTGTGG TTACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga
1921tt

Download FASTA (ORF) (Full)