Transcript: Human XR_001736906.2

PREDICTED: Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZMPSTE24 (10269)
Length:
3156
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001736906.2
NBCI Gene record:
ZMPSTE24 (10269)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001736906.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050428 CGACTCTATCAACTGGATAAA pLKO.1 396 3UTR 100% 13.200 18.480 N ZMPSTE24 n/a
2 TRCN0000050431 CTTTCCTTTGACGAAGGTGTA pLKO.1 932 3UTR 100% 4.050 3.240 N ZMPSTE24 n/a
3 TRCN0000306941 CAGCGGCAGAGAAGGATATAT pLKO_005 309 3UTR 100% 15.000 10.500 N ZMPSTE24 n/a
4 TRCN0000294126 TCACAATCTATGCTGATTATA pLKO_005 829 3UTR 100% 15.000 10.500 N ZMPSTE24 n/a
5 TRCN0000294124 TGGTAAGGCCAATGTTATTTA pLKO_005 2306 3UTR 100% 15.000 10.500 N ZMPSTE24 n/a
6 TRCN0000306867 ACCCACTCTTATTGGACTATT pLKO_005 1533 3UTR 100% 13.200 9.240 N ZMPSTE24 n/a
7 TRCN0000050430 CACCTTACAATGAGGTTCTTT pLKO.1 1577 3UTR 100% 5.625 3.938 N ZMPSTE24 n/a
8 TRCN0000050432 GATCATGGATTCTGAAACATT pLKO.1 365 3UTR 100% 5.625 3.938 N ZMPSTE24 n/a
9 TRCN0000031074 GCCTGGCTGTTCACATTAGTT pLKO.1 792 3UTR 100% 5.625 3.938 N Zmpste24 n/a
10 TRCN0000050429 CCCTGTTTCTGACTGGTTGTT pLKO.1 1722 3UTR 100% 4.950 3.465 N ZMPSTE24 n/a
11 TRCN0000286801 CCCTGTTTCTGACTGGTTGTT pLKO_005 1722 3UTR 100% 4.950 3.465 N ZMPSTE24 n/a
12 TRCN0000031075 CCAAGAAACTTGGGAAGGCTA pLKO.1 1652 3UTR 100% 2.640 1.848 N Zmpste24 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001736906.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14041 pDONR223 100% 45% None (many diffs) n/a
2 ccsbBroad304_14041 pLX_304 0% 45% V5 (not translated due to frame shift) (many diffs) n/a
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