Construct: ORF ccsbBroad304_14041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004628.1_s304c1, BRDN0000406719
- Derived from:
- ccsbBroadEn_14041
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZMPSTE24 (10269)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14041
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10269 | ZMPSTE24 | zinc metallopeptidase STE24 | NM_005857.5 | 99.7% | 99.1% | (many diffs) |
| 2 | human | 10269 | ZMPSTE24 | zinc metallopeptidase STE24 | XR_001736906.2 | 45% | (many diffs) | |
| 3 | mouse | 230709 | Zmpste24 | zinc metallopeptidase, STE24 | NM_172700.2 | 89.8% | 92.8% | (many diffs) |
| 4 | mouse | 230709 | Zmpste24 | zinc metallopeptidase, STE24 | XM_006503019.1 | 86.3% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaagag 61 ttggcatggg gatgtgggcg tcgctggacg ctttgtggga gatgccggcc gagaagcgta 121 tcttcggggc cgtgctgctc ttttcctgga cagtgtatct ttgggagacc ttcctagcac 181 agcggcagag aaggatatat aaaacaacaa ctcatgtacc accggagtta ggacagatca 241 tggattctga aacatttgag aaatctcgac tctatcaact ggataaaagc actttcagct 301 tctggtcagg actctattca gagactgaag gcactcttat tcttctcttt ggaggaatac 361 cttatctctg gagactttct ggacggttct gtggttatgc tggctttgga ccagaatatg 421 agatcactca gtccctggtg tttctgctgt tggctacact tttcagtgca ttggctggtt 481 tgccatggag tctttataat acttttgtga tagaagaaaa acatggcttc aatcaacaga 541 ctttggggtt cttcatgaaa gatgcaatca agaaatttgt tgtgactcag tgcattttgt 601 tgcctgtgtc ttcacttcta ctttacatta ttaaaattgg gggtgactat ttttttattt 661 atgcctggct gttcacatta gttgtgtctc tggttcttgt cacaatctat gctgattata 721 ttgccccttt atttgacaaa ttcacacctc tgcctgaggg aaagcttaaa gaagaaattg 781 aagtaatggc aaagagtatt gactttcctt tgacgaaggt gtatgttgtg gaaggatcta 841 aacgctcttc ccacagcaat gcttattttt atggcttctt caagaacaag cgaatagttt 901 tgtttgacac tctactagaa gagtactctg tactaaacaa agacatccag gaggattctg 961 gcatggaacc ccgcaatgag gaagaaggga acagtgaaga aataaaagct aaagttaaaa 1021 ataagaaaca aggatgtaaa aatgaggagg tactcgctgt actaggccat gaactggggc 1081 actggaagtt gggacataca gtcaaaaata tcattattag ccagatgaat tctttcctgt 1141 gttttttttt atttgctgta ttaattggtc gaaaggagct ttttgctgca tttggttttt 1201 atgatagcca acccactctt attggactat tgatcatctt ccagtttatt ttttcacctt 1261 acaatgaggt tctttctttt tgcctaacag tcctaagccg cagatttgag tttcaagctg 1321 atgcatttgc caagaaactt gggaaggcta aagacttata ttctgcttta atcaaactta 1381 acaaagataa cttgggattc cctgtttctg actggttgtt ctcaatgtgg cattattctc 1441 atcctccact gctagagaga cttcaagctt tgaaaactat gaacaacaca gcccaacctt 1501 cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta 1561 gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta