Transcript: Human XR_001745489.1

PREDICTED: Homo sapiens dihydropyrimidinase (DPYS), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DPYS (1807)
Length:
2588
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001745489.1
NBCI Gene record:
DPYS (1807)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001745489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414308 AGATCGGATGTCCGTAATATG pLKO_005 1213 3UTR 100% 13.200 18.480 N DPYS n/a
2 TRCN0000435308 TGTGGAGCGTGCACCCTATAA pLKO_005 2060 3UTR 100% 13.200 18.480 N DPYS n/a
3 TRCN0000433366 TCAACTCCCTAGGATCCTTTA pLKO_005 2252 3UTR 100% 10.800 15.120 N DPYS n/a
4 TRCN0000046743 CAAGATGTTTATGGCCTATAA pLKO.1 628 3UTR 100% 13.200 9.240 N DPYS n/a
5 TRCN0000046745 CACCACCATGATTATTGATTT pLKO.1 436 3UTR 100% 13.200 9.240 N DPYS n/a
6 TRCN0000046747 TGTGGCAGTTACCAGCACAAA pLKO.1 1276 3UTR 100% 4.950 3.465 N DPYS n/a
7 TRCN0000046744 CTAATGATGATCTAACCACAA pLKO.1 1101 3UTR 100% 4.050 2.835 N DPYS n/a
8 TRCN0000046746 GTGACTATTTCAAGAGGCAAA pLKO.1 1914 3UTR 100% 4.050 2.835 N DPYS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001745489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00460 pDONR223 100% 60.1% None 1_154del;1388_1832del;2157_2588del n/a
2 ccsbBroad304_00460 pLX_304 0% 60.1% V5 1_154del;1388_1832del;2157_2588del n/a
3 TRCN0000472173 GGCGCAGTCATACATCTACTTTAC pLX_317 31.3% 60.1% V5 1_154del;1388_1832del;2157_2588del n/a
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