Construct: ORF TRCN0000472173

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014023.1_s317c1
Derived from:
ccsbBroadEn_00460
DNA Barcode:
GGCGCAGTCATACATCTACTTTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DPYS (1807)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472173
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1807 DPYS dihydropyrimidinase NM_001385.3 100% 100%
2 human 1807 DPYS dihydropyrimidinase XM_005250818.3 93.5% 93.5% 1233_1340del
3 human 1807 DPYS dihydropyrimidinase XM_024447087.1 93.5% 93.5% 1233_1340del
4 human 1807 DPYS dihydropyrimidinase XM_011516903.3 86.6% 86.6% 1233_1340del;1551_1552ins114
5 human 1807 DPYS dihydropyrimidinase XM_006716518.3 83.9% 83.9% 262_263ins159;1074_1181del
6 human 1807 DPYS dihydropyrimidinase XM_017013167.2 82.5% 80.3% (many diffs)
7 human 1807 DPYS dihydropyrimidinase XR_001745490.2 62.7% 1_154del;1388_1724del;2049_2480del
8 human 1807 DPYS dihydropyrimidinase XR_001745489.1 60.1% 1_154del;1388_1832del;2157_2588del
9 mouse 64705 Dpys dihydropyrimidinase NM_001164466.1 85.8% 88.4% (many diffs)
10 mouse 64705 Dpys dihydropyrimidinase NM_022722.3 85.8% 88.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1623
ORF length:
1557
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgccctcg cggctcctga tccgcggggg tcgcgtggtc aacgatgact
121tctcggaggt ggccgacgtg ctggtggagg acggcgtggt gcgggcactc gggcacgacc
181tgctgcctcc cgggggcgct cctgcggggc tgcgggtcct cgacgccgcc ggcaagctcg
241tcctgcccgg aggcatcgac acacacacgc acatgcagtt ccccttcatg ggctcgcggt
301ccatcgacga cttccaccag ggcaccaagg ctgctctctc aggaggcacc accatgatta
361ttgatttcgc cattcctcag aaaggtggct ccctcattga ggccttcgag acctggcgaa
421gctgggctga tcccaaagtt tgctgcgact acagccttca tgtggcagtg acgtggtgga
481gtgaccaggt taaagaagaa atgaaaatcc ttgtgcaaga taaaggtgtt aactctttca
541agatgtttat ggcctataaa gatctgtaca tggtgacaga cctggagctg tacgaagcct
601tctctcggtg caaggaaatt ggagcaattg cccaggtcca tgcggaaaat ggagacttaa
661ttgcagaggg agcaaagaag atgttggctc tggggataac aggccctgag ggccacgagc
721tgtgccgccc agaggcagtg gaggcagagg ccacgctgag agccatcacc atagccagcg
781ctgtgaactg tcctctctac attgtgcatg tgatgagcaa gtctgcagct aaggtgatag
841cggatgcaag gagagatggg aaggtggtct atggtgaacc catagcagcc agtcttggca
901cagatggcac tcactactgg aataaagaat ggcaccatgc agcccaccat gtcatgggtc
961cacctttgcg accagacccc tcaacacccg acttcctcat gaatctgttg gctaatgatg
1021atctaaccac aacagggact gataactgca ctttcaacac ctgccagaaa gctcttggga
1081aggatgattt taccaagatc cccaatgggg tgaatggtgt tgaagatcgg atgtccgtaA
1141TATGGGAAAA AGGCGTGCAT AGTGGTAAAA TGGATGAAAA CAGATTTGTG GCAGTTACCA
1201GCACAAATGC AGCCAAAATT TTTAATCTCT ATCCAAGAAA AGGAAGAATA GCTGTAGGAT
1261CAGATGCTGA CATTGTTATT TGGGACCCAA AAGGCACAAG GACTATCTCA GCAAAAACTC
1321ATCATCAGGC TGTTAACTTC AACATTTTCG AGGGCATGGT TTGCCACGGG GTGCCCCTTG
1381TGACTATTTC AAGAGGCAAA GTGGTATATG AAGCCGGAGT GTTCAGTGTC ACGGCAGGAG
1441ATGGGAAGTT TATTCCTCGA AAACCATTTG CTGAATATAT TTACAAACGA ATAAAGCAGC
1501GAGACCGGAC TTGCACACCT ACCCCTGTGG AGCGTGCACC CTATAAGGGA GAAGTCGCCA
1561CACTGAAATC CAGAGTGACA AAAGAAGATG CCACAGCAGG GACCAGGAAA CAGGCCCACC
1621CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1681TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1741GGCTTTATAT ATCTTGTGGA AAGGACGAGG CGCAGTCATA CATCTACTTT ACACGCGTTA
1801AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)