Transcript: Mouse XR_001781837.1

PREDICTED: Mus musculus DiGeorge syndrome critical region gene 14 (Dgcr14), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dgcr14 (27886)
Length:
2969
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001781837.1
NBCI Gene record:
Dgcr14 (27886)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001781837.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000123620 GCCGAGACAGATAGTACATAA pLKO.1 820 3UTR 100% 13.200 18.480 N Dgcr14 n/a
2 TRCN0000324313 GCCGAGACAGATAGTACATAA pLKO_005 820 3UTR 100% 13.200 18.480 N Dgcr14 n/a
3 TRCN0000123619 CCACAAATATCTAGTGTTGTT pLKO.1 2815 3UTR 100% 4.950 3.960 N Dgcr14 n/a
4 TRCN0000324312 CCACAAATATCTAGTGTTGTT pLKO_005 2815 3UTR 100% 4.950 3.960 N Dgcr14 n/a
5 TRCN0000123621 CCTGGATGAAGAAGAGTACAT pLKO.1 182 3UTR 100% 4.950 3.465 N Dgcr14 n/a
6 TRCN0000324244 CCTGGATGAAGAAGAGTACAT pLKO_005 182 3UTR 100% 4.950 3.465 N Dgcr14 n/a
7 TRCN0000123623 GAAGAGTACATCGAGGGACTT pLKO.1 192 3UTR 100% 4.050 2.835 N Dgcr14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001781837.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01872 pDONR223 100% 41.4% None (many diffs) n/a
2 ccsbBroad304_01872 pLX_304 0% 41.4% V5 (many diffs) n/a
3 TRCN0000479383 ACCGCCCAAGATGCCGCATAAGAC pLX_317 12.5% 41.4% V5 (many diffs) n/a
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