Construct: ORF TRCN0000479383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002966.1_s317c1
- Derived from:
- ccsbBroadEn_01872
- DNA Barcode:
- ACCGCCCAAGATGCCGCATAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESS2 (8220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8220 | ESS2 | ess-2 splicing factor homolog | NM_022719.3 | 100% | 100% | |
| 2 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_005261282.4 | 99.7% | 99.7% | 400_402delGCA |
| 3 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_006724331.3 | 83.4% | 83.4% | 0_1ins237 |
| 4 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_017028958.1 | 83.4% | 83.4% | 0_1ins237 |
| 5 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_006724329.3 | 83.2% | 83.2% | 0_1ins237;163_165delGCA |
| 6 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_006724330.3 | 83.2% | 83.2% | 0_1ins237;163_165delGCA |
| 7 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XR_937926.2 | 76.9% | (many diffs) | |
| 8 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XR_002958714.1 | 74.4% | 1_86del;907_908ins103;1412_1676del | |
| 9 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_011530403.1 | 59.4% | 58% | (many diffs) |
| 10 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_011530404.2 | 57.6% | 57.4% | 400_402delGCA;823_824insAGCACAAACAGGGCA;828_829ins588 |
| 11 | human | 8220 | ESS2 | ess-2 splicing factor homolog | XM_017028959.1 | 43.8% | 36.2% | (many diffs) |
| 12 | human | 8220 | ESS2 | ess-2 splicing factor homolog | NR_134304.2 | 25.4% | (many diffs) | |
| 13 | mouse | 27886 | Dgcr14 | DiGeorge syndrome critical ... | NM_001081633.1 | 85.9% | 92.6% | (many diffs) |
| 14 | mouse | 27886 | Dgcr14 | DiGeorge syndrome critical ... | NM_022408.2 | 85.7% | 92.5% | (many diffs) |
| 15 | mouse | 27886 | Dgcr14 | DiGeorge syndrome critical ... | XM_011245930.2 | 72.7% | 79% | (many diffs) |
| 16 | mouse | 27886 | Dgcr14 | DiGeorge syndrome critical ... | XM_011245931.1 | 46% | 51.2% | (many diffs) |
| 17 | mouse | 27886 | Dgcr14 | DiGeorge syndrome critical ... | XR_001781837.1 | 41.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacgccgggc gcatcagcgt cgtccttgtt gcttcccgcc gcgtccaggc 121 ccccgaggaa gcgcgaggcg ggagaggctg gggctgcgac gagcaagcag cgggtcctgg 181 acgaggaaga gtatatcgag ggcctccaga cggtcatcca aagggatttc tttcctgatg 241 tggagaagct ccaggcacag aaggagtacc tggaagccga ggagaatgga gacttggaac 301 ggatgcgcca gattgccatc aagtttggct ctgccttggg caagatgtcc cgggagcccc 361 cgccacccta tgtgactcca gccacatttg aaacccctga ggtgcatgca ggcactggag 421 tggtgggcaa caagcccagg ccccgcggcc gaggcctgga ggatggagag gctggagagg 481 aggaggagaa ggagccgctg cccagcctag atgtcttcct gagccgctac acgagtgagg 541 acaatgcctc cttccaggag atcatggagg tggccaagga gagaagccgg gcacgccacg 601 cttggctcta ccaggctgag gaagagtttg agaagaggca gaaagataat ctcgaactcc 661 cgtcagcaga gcaccaggcc atcgagagca gccaggccag tgtggagacc tggaagtaca 721 aggccaagaa ttccctcatg tactatccag agggtgtccc tgacgaggag cagctgttta 781 agaagccccg gcaggtggta cataagaaca cgcgcttcct tagggacccc ttcagccaag 841 ccctgagcag gtgccagctc cagcaggcag ccgccctcaa tgcccagcac aaacagggca 901 aggtgggccc cgatggcaag gagctgatcc cccaggagtc ccctcgagtg ggtggatttg 961 gatttgttgc cactccttcc cctgcccctg gtgtgaacga gtccccgatg atgacctggg 1021 gggaggttga gaacacaccc ttgagagttg aagggtcgga aacgccctac gtggacagga 1081 cacccggccc agcttttaag atcctggagc caggccgcag ggagcggctg ggtctgaaga 1141 tggccaacga ggccgctgcc aagaaccggg ccaagaagca ggaagccttg cggagagtga 1201 cggagaatct ggccagcctc acccccaaag gcctgagccc agccatgtcg ccagccctac 1261 agcgccttgt gagcaggacg gccagcaagt acacagaccg ggccctgcgg gccagctaca 1321 caCCATCCCC AGCACGCTCC ACCCACCTCA AGACCCCGGC CAGTGGGCTG CAGACCCCCA 1381 CAAGCACACC GGCGCCTGGC TCTGCCACAC GCACCCCTCT CACACAGGAC CCGGCCTCCA 1441 TCACGGACAA CCTGCTGCAG CTCCCTGCCC GGCGCAAAGC TTCGGACTTC TTTTACCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAA CCGCCCAAGA TGCCGCATAA GACACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att