Transcript: Human XR_002957833.1

PREDICTED: Homo sapiens acyl-CoA synthetase medium chain family member 3 (ACSM3), transcript variant X8, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACSM3 (6296)
Length:
3482
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957833.1
NBCI Gene record:
ACSM3 (6296)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957833.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434770 TATCCTCTGGCTATCGAATTG pLKO_005 3290 3UTR 100% 10.800 15.120 N ACSM3 n/a
2 TRCN0000083242 CCTGCTTTCGATGTTAAGATT pLKO.1 1739 3UTR 100% 5.625 7.875 N ACSM3 n/a
3 TRCN0000419964 CAAAGAGGAGATCGGGTAATT pLKO_005 884 3UTR 100% 13.200 10.560 N ACSM3 n/a
4 TRCN0000420216 CAGACTGAAACGGTGCTAATC pLKO_005 1664 3UTR 100% 10.800 8.640 N ACSM3 n/a
5 TRCN0000083241 CCTGGACCAATGGACTGATAA pLKO.1 724 3UTR 100% 13.200 9.240 N ACSM3 n/a
6 TRCN0000083238 GCTGGAAAGAAACCTTCAAAT pLKO.1 752 3UTR 100% 13.200 9.240 N ACSM3 n/a
7 TRCN0000419608 TATGGATAAAGATGGGTATTT pLKO_005 3237 3UTR 100% 13.200 9.240 N ACSM3 n/a
8 TRCN0000083240 CCCTCAGATGTGATGTGGAAT pLKO.1 1334 3UTR 100% 4.950 3.465 N ACSM3 n/a
9 TRCN0000083239 CCTGATTACAAGTCACATGAT pLKO.1 3427 3UTR 100% 4.950 3.465 N ACSM3 n/a
10 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3002 3UTR 100% 4.950 2.475 Y DCAF11 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957833.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11116 pDONR223 100% 36.2% None (many diffs) n/a
2 ccsbBroad304_11116 pLX_304 0% 36.2% V5 (many diffs) n/a
3 TRCN0000492316 CCACTCTCCTATCCTCATCATTTA pLX_317 23.6% 36.2% V5 (many diffs) n/a
Download CSV