Construct: ORF TRCN0000492316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000373.2_s317c1
- Derived from:
- ccsbBroadEn_11116
- DNA Barcode:
- CCACTCTCCTATCCTCATCATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSM3 (6296)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | NM_202000.2 | 98.1% | 98.1% | 1_24del |
| 2 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_024450371.1 | 73.6% | 68.7% | (many diffs) |
| 3 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | NM_005622.4 | 72.2% | 67.4% | (many diffs) |
| 4 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_024450370.1 | 72.2% | 67.4% | (many diffs) |
| 5 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_017023523.2 | 71% | 66.3% | (many diffs) |
| 6 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_024450369.1 | 71% | 66.3% | (many diffs) |
| 7 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_011545911.2 | 68% | 63.5% | (many diffs) |
| 8 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_024450368.1 | 68% | 63.5% | (many diffs) |
| 9 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XM_024450367.1 | 66.9% | 62.5% | (many diffs) |
| 10 | human | 6296 | ACSM3 | acyl-CoA synthetase medium ... | XR_002957833.1 | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcatgct acgtcatgcc aagtgttttc agcgcctagc aatttttggt tctgtgaggg 121 cactgcataa agataataga acagcaaccc ctcagaattt ctccaactat gaatccatga 181 aacaggactt caaactgggg attccagagt atttcaactt tgctaaagat gtcctggacc 241 aatggactga taaggaaaag gctggaaaga aaccttcaaa tccagccttc tggtggatca 301 acagaaatgg agaagagatg cgatggagtt ttgaggaact gggatctctg tccagaaaat 361 ttgccaatat actttcagaa gcctgttccc tacaaagagg agatcgggta attctgattc 421 tgcccagggt cccagagtgg tggcttgcaa atgtggcctg tctgcgaaca gggacagttt 481 taattccagg aaccactcag ctgacccaga aagacattct ctacagacta caatcttcaa 541 aagcaaactg cattatcacc aatgatgttt tagccccagc agtagacgct gttgcatcca 601 aatgtgaaaa tctgcactcc aagctgattg tatcagagaa ctccagagag gggtggggga 661 acctcaagga gttgatgaaa catgccagtg acagccacac ctgtgtgaag acaaaacaca 721 atgagatcat ggccatattc tttaccagtg gaacaagtgg atatccgaaa atgactgcac 781 acacccacag cagttttggt ttaggattat ctgtaaatgg aaggttctgg ctagatttga 841 caccctcaga tgtgatgtgg aatacctcag atacgggctg ggcaaagtct gcatggagta 901 gtgttttttc tccgtggatc cagggagcat gtgtattcac acaccattta ccccgttttg 961 agccgacttc tatcttgcaa acactctcca agtaccccat cacagtcttc tgttcagcac 1021 caactgtata ccgaatgctt gtacagaatg atataaCCAG CTATAAGTTT AAAAGCTTAA 1081 AGCACTGTGT GAGTGCTGGG GAACCAATTA CCCCTGACGT GACTGAAAAA TGGAGAAACA 1141 AGACGGGCCT GGATATCTAC GAAGGATATG GACAGACTGA AACGGTGCTA ATCTGTGGAA 1201 ATTTTAAGGG AATGAAAATT AAACCTGGCT CAATGGGAAA ACCTTCTCCT GCTTTCGATG 1261 TTAAGGTTTG CACATCCCCT TCCAGGAGAA TGTTTAACAA CCCAATCTGT ACACTACCTA 1321 CCTACCGCTT ACCCCCATAT AAACTTTCTT TGTTATGCCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGCCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ACCACTCTCC TATCCTCATC ATTTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt