Transcript: Human XR_923058.1

PREDICTED: Homo sapiens kynureninase (KYNU), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KYNU (8942)
Length:
1767
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_923058.1
NBCI Gene record:
KYNU (8942)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_923058.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000051372 GCACTTTAATATTCCTGCCAT pLKO.1 779 3UTR 100% 2.640 2.112 N KYNU n/a
2 TRCN0000431602 TGGTGTTCCTACAAGTATTTA pLKO_005 906 3UTR 100% 15.000 10.500 N KYNU n/a
3 TRCN0000430390 ACTACAACTTCACGGACTTAA pLKO_005 617 3UTR 100% 13.200 9.240 N KYNU n/a
4 TRCN0000051370 CCTCCAGTTGATTTATCATTA pLKO.1 255 3UTR 100% 13.200 9.240 N KYNU n/a
5 TRCN0000424571 GGACAAGCGAAGGGTTGTTAT pLKO_005 810 3UTR 100% 13.200 9.240 N KYNU n/a
6 TRCN0000418120 ACCTGATCAAGCATAACTATG pLKO_005 1339 3UTR 100% 10.800 7.560 N KYNU n/a
7 TRCN0000051368 CCTGCATTAGTGGGATGGTTT pLKO.1 990 3UTR 100% 4.950 3.465 N KYNU n/a
8 TRCN0000051369 CCTCTCTATAATTCTTTCCAT pLKO.1 1545 3UTR 100% 3.000 2.100 N KYNU n/a
9 TRCN0000051371 GCCATCTATTTCTTGGGAAAT pLKO.1 294 3UTR 100% 1.080 0.756 N KYNU n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_923058.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02052 pDONR223 100% 51.8% None (many diffs) n/a
2 ccsbBroad304_02052 pLX_304 0% 51.8% V5 (many diffs) n/a
3 TRCN0000472118 GGGCGCATCCCAGTACGAATTCCG pLX_317 45.9% 51.8% V5 (many diffs) n/a
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